The Recombinant Phospho-Chk2 (Thr68) Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C110219) against Chk2 (Thr68). In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC, IF, IP and ELISA applications. It has been validated by WB analysis of NIH-3T3. The target is localized to the The protein is primarily localized in the nucleus. Specifically, isoform 10 is present throughout the cell, while other isoforms are concentrated in the nucleoplasm and PML bodies. Notably, it is recruited into PML bodies in conjunction with TP53.
CDS, CDS 1, CDS1, Cds1 homolog, Checkpoint kinase 2, Checkpoint like protein CHK2, CHEK, CHEK 2, CHEK2, CHK, Chk 2, CHK2, CHK2 checkpoint homolog, CHK2 checkpoint homolog (S. pombe), hCds, hCds1, HuCds, HuCds 1, HuCds1, LFS, LFS 2, LFS2, PP1425, RAD, RAD 53, RAD53, Rad53 homolog, Serine/threonine protein kinase Chk2, Serine/threonine-protein kinase Chk2
Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic peptide corresponding to residues around (Thr68) of Human Phospho-Chk2
Applications:
WBIHCIFIPELISA
Clonality:
Recombinant Monoclonal
Clone No:
C110219
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
Chk2 (Thr68)
Conjugation:
Unconjugated
Observed MW:
61 kDa
Calculated MW:
61 kDa
Recommended Dilution:
IHC 1:200-1000; WB 1:2000-10000; IF 1:200-1000; ELISA 1:5000-20000; IP 1:50-200
UniProt:
O96017
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Western Blot with Recombinant Phospho-Chk2 (Thr68) Monoclonal Antibody at dilution of 1:1000 dilution. Lane A: NIH-3T3 whole cell lysate. Observed-MW:61 kDa Calculated-MW:61 kDa
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