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Recombinant Phospho-eIF2α (Ser51) Monoclonal Antibody (RMEA0793)

Datasheet MSDS
SKU RMEA0793
Product Type Antibody
Reactivity Human, Mouse, Rat
Applications WB, IHC, IF, ELISA
Host Species Rabbit
Clonality Monoclonal
Clone C110791
Conjugate Unconjugated
$540 - $738

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Description

system_update_altDatasheetsystem_update_altMSDS

Recombinant Phospho-eIF2α (Ser51) Monoclonal Antibody

The Recombinant Phospho-eIF2α (Ser51) Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C110791) against eIF2α (Ser51). The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.

This antibody is suitable for WB, IHC, IF and ELISA applications. It has been validated by WB analysis of Hela and IHC analysis of Human hepatocellular carcinoma. The target is localized to the cytoplasm, stress granule, Colocalizes with NANOS3 in the stress granules.

Product Name:Recombinant Phospho-eIF2α (Ser51) Monoclonal Antibody
SKU:RMEA0793
Size:50 µL, 100 µL
Antibody Type:Recombinant Monoclonal Antibody
Synonyms:EIF, EIF 2, EIF 2 alpha, EIF 2A, EIF 2alpha, EIF-2, eIF-2-alpha, EIF-2A, EIF-2alpha, EIF2, EIF2 alpha, EIF2A, EIF2S, EIF2S1, Eukaryotic translation initiation factor 2 subunit 1, Eukaryotic translation initiation factor 2 subunit 1 alpha, Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa, Eukaryotic translation initiation factor 2 subunit alpha, IF2A
Reactivity:Human, Mouse, Rat
Immunogen:A synthetic peptide corresponding to residues around (Ser51) of Human Phospho-eIF2α
Applications:WBIHCIFELISA
Clonality:Recombinant Monoclonal
Clone No:C110791
Host:Rabbit
Isotype:Rabbit IgG
Target:eIF2α (Ser51)
Conjugation:Unconjugated
Observed MW:36 kDa
Calculated MW:36 kDa
Recommended Dilution:IHC 1:100-1000; WB 1:2000-10000; IF 1:200-1000; ELISA 1:5000-20000
UniProt:P05198
Purification:Protein A
Concentration:0.2 mg/mL
Buffer:PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Product ImageImmunohistochemistry of paraffin-embedded human hepatocellular carcinoma using Recombinant Phospho- eIF2α (Ser51) Monoclonal Antibody at dilution of 1:200.
Product ImageWestern Blot with Recombinant Phospho-eIF2α (Ser51) Monoclonal Antibody at dilution of 1:1000 dilution. Lane A: Hela. Observed-MW:36 kDa Calculated-MW:36 kDa
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