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Recombinant Phospho-GSK3 beta (Ser9) Monoclonal Antibody (AGEL5423)

Datasheet MSDS
SKU AGEL5423
Product Type Antibody
Reactivity Human, Mouse
Applications WB, IHC, IF, ELISA
Host Species Rabbit
Clonality Monoclonal
Clone C210104
Conjugate Unconjugated
$540 - $738

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Description

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Recombinant Phospho-GSK3 beta (Ser9) Monoclonal Antibody

The Recombinant Phospho-GSK3 beta (Ser9) Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C210104) against GSK3 beta (Ser9). The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human and Mouse samples.

This antibody is suitable for WB, IHC, IF and ELISA applications. It has been validated by WB analysis of HL60, HUVEC, Human CAF, HCT116, A549, Jurkat, HT22, THP-1, HK-2, PC-12, 3T3-L1, PC-3, U-14, 293F, Hela, Hela starved of serum overnight and then treated with 20% fetal bovine serum and Calyculin A (100 nM) for 15 minutes, IHC analysis of Human breast carcinoma and IF analysis of HEK293. The target is localized to the cell membrane.

Product Name:Recombinant Phospho-GSK3 beta (Ser9) Monoclonal Antibody
SKU:AGEL5423
Size:50 µL, 100 µL
Antibody Type:Recombinant Monoclonal Antibody
Synonyms:Glycogen Synthase Kinase 3 Beta, Glycogen synthase kinase-3 beta, GSK 3 beta, GSK-3 beta, gsk-3β, GSK3B, GSK3beta isoform, Serine/threonine-protein kinase GSK3B
Reactivity:Human, Mouse
Immunogen:A synthetic peptide corresponding to residues around Ser9 of Human Phospho-GSK3 beta
Applications:WBIHCIFELISA
Clonality:Recombinant Monoclonal
Clone No:C210104
Host:Rabbit
Isotype:Rabbit IgG
Target:GSK3 beta (Ser9)
Conjugation:Unconjugated
Observed MW:46 kDa
Calculated MW:46 kDa
Recommended Dilution:IHC 1:200-1000; WB 1:1000-5000; IF 1:200-1000; ELISA 1:5000-20000
UniProt:P49841, Q9WV60, P18266
Purification:Protein A
Concentration:1 mg/mL
Buffer:0.2 µm filtered solution in PBS
Storage:Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Product ImageHuman breast carcinoma was stained with Anti-GSK3 β (phospho Ser9) rabbit antibody
Product ImageHuman breast carcinoma was stained with Anti-GSK3 β (phospho Ser9) rabbit antibody
Product ImageVarious whole cell lysates were separated by 4-20% SDS- 4℃ PAGE, and the primary antibody was used at over night with a 1:2500 dilution. The Dylight 800-conjugated Goat anti-Rabbit antibody was used to detect the antibody. Lane1: HL60 cell Lane2: HUVEC cell Lane3: Human CAF Lane4: HCT116 Lane5: A549 Lane6: Jurkat Lane7: HT22 Lane8: THP-1 Lane9: HK-2 Lane10: PC-12 Lane11: 3T3-L1 Lane12: PC-3 Lane13: U-14 Lane14: 293F Predicted band size: 46kDa Observed band size: 46kDa
Product ImageVarious whole cell lysates were separated by 4-20% SDS- PAGE, and the membrane was blotted with anti-GSK3 β (phospho Ser9) antibody. The HRP-conjugated Goat anti- Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: Hela Lane 2: Hela starved of serum overnight and then treated with 20% fetal bovine serum and Calyculin A (100 nM) for 15 minutes Predicted band size: 46kDa Observed band size: 46kDa Immunofluorescence analysis of HEK293. Picture A: GSK3 β (Phospho Ser9) antibody (red). Picture B: DAPI (blue). Picture C: Merge of A+B
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