The Recombinant Phospho-GSK3 β (Ser9) Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C210685) against GSK3 β (Ser9). The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human and Mouse samples.
This antibody is suitable for WB, IHC, IF and ELISA applications. It has been validated by WB analysis of Hela and IHC analysis of Human breast cancer tissue. The target is localized to the cytoplasmic, membranous.
A synthetic peptide corresponding to residues around (Ser9) of Human Phospho-GSK3 β
Applications:
WBIHCIFELISA
Clonality:
Recombinant Monoclonal
Clone No:
C210685
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
GSK3 β (Ser9)
Conjugation:
Unconjugated
Observed MW:
46 kDa
Calculated MW:
46 kDa
Recommended Dilution:
IHC 1:200-1000; WB 1:1000-5000; IF 1:200-1000; ELISA 1:5000-20000
UniProt:
P49841
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Immunohistochemistry of paraffin-embedded human breast cancer tissue using Recombinant Phospho-GSK3 β (Ser9) Monoclonal Antibody at dilution of 1:200.
Western Blot with Recombinant Phospho-GSK3 β (Ser9) Monoclonal Antibody at dilution of 1:1000 dilution. Lane A: Hela cells. Observed-MW:46 kDa Calculated-MW:46 kDa