The Recombinant Phospho-HSP27 (Ser78) Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C311708) against HSP27 (Ser78). Hsp27 is one of the small HSPs that are constitutively expressed at different levels in various cell types and tissues. Like other small HSPs, HSP27 is regulated at both the transcriptional and posttranslational levels. In response to stress, the HSP27 expression increases several-fold to confer cellular resistance to the adverse environmental change. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human samples.
This antibody is suitable for WB, IHC, IF and IP applications. It has been validated by WB analysis of HeLa + Anisomycin(-), 2 HeLa+ Anisomycin(+), IHC analysis of Human breast cancer, Human cervical cancer, Human tonsil, IF analysis of A: HeLa, B HeLa+ Anisomycin(+) and IP analysis of HeLa+Anisomycin (250ng/ml, 30min) cells extracts. The target is localized to the cytoplasm, cytoskeleton, nucleus.
28 kDa heat shock protein, CMT2F, DKFZp586P1322, epididymis secretory protein Li 102, Estrogen regulated 24 kDa protein, Estrogen-regulated 24 kDa protein, Heat shock 25kDa protein 1, Heat shock 27 kDa protein, Heat shock 27kD protein 1, Heat shock 27kDa protein, Heat shock 27kDa protein 1, Heat shock 28kDa protein 1, Heat Shock Protein 27, Heat shock protein beta 1, Heat shock protein beta-1, heat shock protein family B (small) member 1, HEL-S, HEL-S-102, HMN2B, HS.76067, HSP, Hsp 25, HSP 27, Hsp 28, Hsp B1, Hsp25, HSP27, HSP28, HSPB, HSPB1, SRP, SRP27, Stress responsive protein 27, Stress-responsive protein 27
Reactivity:
Human
Immunogen:
phosphorylated human HSP27 (Ser78) peptide
Applications:
WBIHCIFIP
Clonality:
Recombinant Monoclonal
Clone No:
C311708
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
HSP27 (Ser78)
Conjugation:
Unconjugated
Observed MW:
27 kDa
Calculated MW:
23 kDa
Recommended Dilution:
WB 1:500-1000; IHC 1:100-500; IF 1:50; IP 1:50-100
UniProt:
P04792
Purification:
Protein A
Concentration:
1 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Immunofluorescent analysis of (100% Ice-cold methanol) fixed A: HeLa, B HeLa+ Anisomycin(+) cells using anti- Phospho-HSP27 (Ser78) Monoclonal Antibody at dilution of 1:50.
Immunohistochemistry of paraffin-embedded Human breast cancer using Phospho-HSP27 (Ser78) Monoclonal Antibody at dilution of 1:500.
Immunohistochemistry of paraffin-embedded Human cervical cancer using Phospho-HSP27 (Ser78) Monoclonal Antibody at dilution of 1:500.
Immunohistochemistry of paraffin-embedded Human tonsil using Phospho-HSP27 (Ser78) Monoclonal Antibody at dilution of 1:500.
Immunoprecipitation analysis using anti-Phospho-HSP27 (Ser78) Monoclonal Antibody. Western blot was performed from the immunoprecipitate using Phospho-HSP27 (Ser78) Monoclonal Antibody at a dilution of 1:100. Lane 1: 5% Input, Lane 2: Phospho-HSP27 Monoclonal Antibody, Lane 3: Rabbit monoclonal IgG Isotype Observed-MW:27 kDa Calculated-MW:23 kDa
Western Blot with Phospho-HSP27 (Ser78) Monoclonal Antibody at dilution of 1:1000. Lane 1: HeLa + Anisomycin(-), 2 HeLa+ Anisomycin(+) Observed-MW:27 kDa Calculated-MW:23 kDa
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