The Recombinant Phospho-IκBα (Ser36) Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C231716) against IκBα (Ser36). The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins. Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB. IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human and Mouse samples.
This antibody is suitable for WB applications. It has been validated by WB analysis of HeLa. The target is localized to the cytoplasm, nucleus.
alpha, EDAID, EDAID2, I kappa B alpha, I-kappa-B-alpha, IkappaBalpha, IKB alpha, IkB-alpha, IKBA, IKBalpha, MAD, MAD 3, MAD-3, MAD3, Major histocompatibility complex enhancer-binding protein MAD3, NF kappa B inhibitor alpha, NF-kappa-B inhibitor alpha, NFKBI, NFKBIA, Nuclear factor of kappa light chain gene enhancer in B cells, Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, pIκBα
Reactivity:
Human, Mouse
Immunogen:
Phosphorylated human IκBα (Ser36) peptide
Applications:
WB
Clonality:
Recombinant Monoclonal
Clone No:
C231716
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
IκBα (Ser36)
Conjugation:
Unconjugated
Observed MW:
39 kDa
Calculated MW:
36 kDa
Recommended Dilution:
WB 1:1000-2000
UniProt:
P25963
Purification:
Protein A
Concentration:
1 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Western Blot with Phospho-IκBα (Ser36) Monoclonal Antibody at dilution of 1:2000. (-): HeLa, (+): HeLa + Calyculin A (100ng/ml, 30min)+TNF-α (20ng/ml, 5min) Observed-MW:39 kDa Calculated-MW:36 kDa
![Anti-Phospho-IKB alpha (Ser36) [R05-4K3] Monoclonal Antibody (AGMB05135)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/276420/680081/anti-phospho-ikb-alpha-ser36-r05-4k3-monoclonal-antibody-agmb05135__09204.1773040946.jpg?c=2 "Anti-Phospho-IKB alpha (Ser36) [R05-4K3] Monoclonal Antibody (AGMB05135)")
