The Recombinant Phospho-MSK1 (Ser360) Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C170640) against MSK1 (Ser360). ATP + a protein = ADP + a phosphoprotein., cofactor:Magnesium., enzyme regulation:Appears to be activated by multiple phosphorylations on threonine and serine residues. ERK1/2 and MAPK14/p38-alpha may play a role in this process., Serine/threonine kinase required for the mitogen or stress-induced phosphorylation of the transcription factors CREB (cAMP response element-binding protein) and ATF1 (activating transcription factor-1). Essential role in the control of RELA transcriptional activity in response to TNF. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IF, IP and ELISA applications. It has been validated by WB analysis of C6. The target is localized to the cytoplasm, nuclear.
90 kDa ribosomal protein S6 kinase 5, EC 2.7.11.1, KS6A5, MGC1911, Mitogen and stress activated protein kinase 1, MSK, MSK1, MSPK, MSPK1, Nuclear Mitogen And Stress Activated Protein Kinase 1, Nuclear mitogen- and stress-activated protein kinase 1, Ribosomal protein S6 kinase 90kD polypeptide 5, Ribosomal protein S6 kinase 90kDa, Ribosomal protein S6 kinase 90kDa polypeptide 5, Ribosomal Protein S6 Kinase Alpha 5, Ribosomal protein S6 kinase alpha-5, RLPK, RPS6KA, RPS6KA5, RSK Like Protein Kinase, RSK-like protein kinase, RSKL, S6K alpha 5, S6K-alpha-5
Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic peptide corresponding to residues around (Ser360) of Human Phospho-MSK1
Applications:
WBIFIPELISA
Clonality:
Recombinant Monoclonal
Clone No:
C170640
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
MSK1 (Ser360)
Conjugation:
Unconjugated
Observed MW:
90 kDa
Calculated MW:
90 kDa
Recommended Dilution:
WB 1:1000-5000; IF 1:200-1000; ELISA 1:5000-20000; IP 1:50-200
UniProt:
O75582
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Western Blot with Recombinant Phospho-MSK1 (Ser360) Monoclonal Antibody at dilution of 1:1000 dilution. Lane A: C6 cells. Observed-MW:90 kDa Calculated-MW:90 kDa