The Recombinant Phospho-ULK1 (Ser757) Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C170283) against ULK1 (Ser757). ATP + a protein = ADP + a phosphoprotein., Involved in axon growth. Plays an essential role in neurite extension of cerebellar granule cells., similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IF, IP and ELISA applications. It has been validated by WB analysis of NIH-3T3. The target is localized to the cytoplasm, cytosol, preautophagosomal structure, under starvation conditions, is localized to puncate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome.
A synthetic peptide corresponding to residues around (Ser757) of Human Phospho-ULK1
Applications:
WBIFIPELISA
Clonality:
Recombinant Monoclonal
Clone No:
C170283
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
ULK1 (Ser757)
Conjugation:
Unconjugated
Observed MW:
150 kDa
Calculated MW:
113 kDa
Recommended Dilution:
WB 1:2000-10000; IF 1:200-1000; ELISA 1:5000-20000; IP 1:50-200
UniProt:
O75385
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Western Blot with Recombinant Phospho-ULK1 (Ser757) Monoclonal Antibody at dilution of 1:1000 dilution. Lane A: NIH-3T3 cell lysate. Observed-MW:150 kDa Calculated-MW:113 kDa
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