The Recombinant Phospho-YAP (Ser127) Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C170285) against YAP (Ser127). This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IF and ELISA applications. It has been validated by WB analysis of Hela. The target is localized to the cytoplasm, nucleus.
A synthetic peptide corresponding to residues around (Ser127) of Human Phospho-YAP
Applications:
WBIFELISA
Clonality:
Recombinant Monoclonal
Clone No:
C170285
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
YAP (Ser127)
Conjugation:
Unconjugated
Observed MW:
55 kDa
Calculated MW:
55 kDa
Recommended Dilution:
WB 1:2000-10000; IF 1:200-1000; ELISA 1:5000-20000
UniProt:
P46937
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Western Blot with Recombinant Phospho-YAP (Ser127) Monoclonal Antibody at dilution of 1:1000 dilution. Lane A: Hela cell lysate. Observed-MW:55 kDa Calculated-MW:55 kDa