The Recombinant PRDM4/PFM1 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C121665) against PRDM4/PFM1. The PRDM family members are characterized by the presence of a PR (positive regulatory) domain and multiple zinc finger (ZnFg) domains. The PR domain defines a family of transcription factors involved in cell differentiation and tumorigenesis. PRDM proteins are either epigenetic modifiers in their own right or else they recruit third party chromatin modifiers - e.g. histone deacetylases (HDACs), histone lysine MTases or histone arginine MTases - to regulate cell type-specific gene expression in various tissues (PRDM4 as an HDAC-associated transcriptional repressor that modulates cell cycle progression. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB and IHC applications. It has been validated by WB analysis of HeLa,?293T, Mouse heart, Rat heart and IHC analysis of Human colon, Human pancreas, Human testis. The target is localized to the nucleus.
Product Name:
Recombinant PRDM4/PFM1 Monoclonal Antibody
SKU:
AGEL6970
Size:
50 µL, 100 µL
Antibody Type:
Recombinant Monoclonal Antibody
Synonyms:
PFM, PFM1, PRDM, PRDM4
Reactivity:
Human, Mouse, Rat
Immunogen:
Peptide. This information is proprietary to PTMab.
Applications:
WBIHC
Clonality:
Recombinant Monoclonal
Clone No:
C121665
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
PRDM4/PFM1
Conjugation:
Unconjugated
Observed MW:
100 kDa
Calculated MW:
88 kDa
Recommended Dilution:
WB 1:1000; IHC 1:500
UniProt:
Q9UKN5
Purification:
Protein A
Concentration:
1 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Immunohistochemistry of paraffin-embedded Human colon using PRDM4/PFM1 Monoclonal Antibody at dilution of 1:500.
Immunohistochemistry of paraffin-embedded Human pancreas using PRDM4/PFM1 Monoclonal Antibody at dilution of 1:500.
Immunohistochemistry of paraffin-embedded Human testis using PRDM4/PFM1 Monoclonal Antibody at dilution of 1:500.
Western Blot with PRDM4/PFM1 Monoclonal Antibody at dilution of 1:1000. Lane 1: HeLa, Lane 2: 293T, Lane 3: Mouse heart, Lane 4: Rat heart Observed-MW:100 kDa Calculated-MW:88 kDa
