Tumor necrosis factor alpha (TNF?) is the prototypic ligand of the TNF superfamily. TNF? forms a homotrimer and functions by activating two types of receptors TNF-R1 (TNF receptor type 1,p55R) and TNF-R2 (TNF receptor type 2,p75R). TNF? is a pleiotropic cytokine that is capable to promote inflammation, to induce apoptotic cell death, and to inhibit tumorigenesis and viral replication. TNF? is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells.
Product Name:
Rabbit TNF-alpha/TNFSF2/TNFa Recombinant Protein (RPES3618)
Measured in a cytotoxicity assay using L?929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 3.5 pg/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Rabbit TNFa is produced by our E.coli expression system and the target gene encoding Val77-Leu235 is expressed.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
17.59 kDa
AP Mol Mass:
18 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB, 300mM NaCl, pH7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
