CD70, a member of the tumor necrosis factor superfamily, is restricted to activated T-and B-lymphocytes and mature dendritic cells. Binding of CD70 to its receptor, CD27, is important in priming, effector functions, differentiation and memory formation of T-cells as well as plasma and memory B-cell generation. Tight control of CD70 expression is required to prevent lethal immunodeficiency. By selective transcription, CD70 is largely confined to activated lymphocytes and dendritic cells (DC). As a type II transmembrane receptor, CD70 is normally expressed on a subset of B, T and NK cells, where it plays a costimulatory role in immune cell activation. Immunohistochemical analysis of CD70 expression in multiple carcinoma types. The restricted expression pattern of CD70 in normal tissues and its widespread expression in various malignancies makes it an attractive target for antibody-based therapeutics. Investigations to exploit CD70 as a cancer target have lead to the identification of potential antibody-based clinical candidates.Immune CheckpointImmune Checkpoint ProteinsImmune Checkpoint Targets Co-stimulatory Immune Checkpoint Targets Immunotherapy Cancer Immunotherapy Targeted Therapy
Product Name:
Rat TNFSF7/CD70 Recombinant Protein (RPES0397)
Product Code:
RPES0397
Size:
50 µg
Species:
Rat
Expressed Host:
HEK293 Cells
Synonyms:
CD70
Accession:
NP_001100348
Sequence:
Gln46-Pro195
Fusion tag:
N-His
Activity:
1.Measured by its binding ability in a functional ELISA.2.Immobilized rat His-CD70 at 10µg/mL (100?L/well) can bind mouse CD27-Fch , the EC50 of mouse CD27-Fch is 10-50ng/mL.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method
Protein Construction:
A DNA sequence encoding the rat CD70 (NP_001100348) (Gln46-Pro195) was expressed, fused with a polyhistidine tag at the N-terminus.
Purity:
> 90 % as determined by SDS-PAGE
Mol Mass:
18.9 kDa
AP Mol Mass:
28 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
