The Recombinant SETD1A Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C141180) against SETD1A. hSET1 is a component of the histone methyltransferase (HMT) complex, which is a member of the COMPASS family, has H3K4 methyltransferase activity, and is closely related to development and tumors. hSET1 can produce mono-, di- and Trimethylation at lys4 (K4) on histone H3. This activity is blocked by the pre-methylation of the transcription repressor K9, indicating that the SET1/ASH2 complex can cause crosstalk between K9 methylation and K4 methylation. SET1 protein may play a role in synaptic function and neuron development. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for IHC, IF and FCM applications. It has been validated by IHC analysis of Human breast cancer, Mouse small intestine, Rat colon, IF analysis of HeLa, MCF-7 and FCM analysis of HeLa. The target is localized to the nucleus.
Product Name:
Recombinant SETD1A Monoclonal Antibody
SKU:
AGEL6485
Size:
50 µL, 100 µL
Antibody Type:
Recombinant Monoclonal Antibody
Synonyms:
Histone-lysine N-methyltransferase SETD1A, hSET1A, KIAA, KIAA0339, KMT2F, Lysine N methyltransferase 2F, Lysine N-methyltransferase 2F, Set, SET domain containing 1A, SET domain containing protein 1A, SET domain-containing protein 1A, Set1, Set1 Ash2 histone methyltransferase complex subunit SET1, Set1/Ash2 histone methyltransferase complex subunit SET1, Set1A, SETD1A
Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant human SETD1A fragment
Applications:
IHCIFFCM
Clonality:
Recombinant Monoclonal
Clone No:
C141180
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
SETD1A
Conjugation:
Unconjugated
Recommended Dilution:
IHC 1:50-100; IF 1:100-500; FCM 1:50-100
UniProt:
O15047
Purification:
Protein A
Concentration:
1 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Flow cytometric analysis of human SETD1A expression on HeLa cells. Cells were stained with purified anti-Human SETD1A, then a Alexa Fluor 488-conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of intact cells.
Immunofluorescent analysis of (100% Ice-cold methanol) fixed HeLa cells using anti-SETD1A Monoclonal Antibody at dilution of 1:500.
Immunofluorescent analysis of (100% Ice-cold methanol) fixed MCF-7 cells using anti-SETD1A Monoclonal Antibody at dilution of 1:500.
Immunohistochemistry of paraffin-embedded Human breast cancer using SETD1A Monoclonal Antibody at dilution of 1:100.
Immunohistochemistry of paraffin-embedded Mouse small intestine using SETD1A Monoclonal Antibody at dilution of 1:100.
Immunohistochemistry of paraffin-embedded Rat colon using SETD1A Monoclonal Antibody at dilution of 1:100.
