The Recombinant SHIP-2 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C311650) against SHIP-2. SH2-containing inositol phosphatase 1 (SHIP1) is a hematopoietic phosphatase that hydrolyzes phosphatidylinositol-3, 4, 5-triphosphate to phosphatidylinositol-3, 4-bisphosphate. SHIP1 is a cytosolic phosphatase with an SH2 domain in its amino terminus and two NPXY Shc binding motifs in its carboxy terminus. Upon receptor cross-linking, SHIP is first recruited to the membrane junction through binding of its SH2 domain to the phospho-tyrosine in the ITIM motif, followed by tyrosine phosphorylation on the NPXY motif. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human samples.
This antibody is suitable for WB, IHC and IF applications. It has been validated by WB analysis of Jurkat (negative control), HeLa, K562, IHC analysis of Human cardiac muscle, Human breast cancer and IF analysis of HeLa. The target is localized to the cytoplasm, nucleus, cell projection.
Peptide. This information is proprietary to PTMab.
Applications:
WBIHCIF
Clonality:
Recombinant Monoclonal
Clone No:
C311650
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
SHIP-2
Conjugation:
Unconjugated
Observed MW:
140 kDa
Calculated MW:
139 kDa
Recommended Dilution:
WB 1:1000; IHC 1:100; IF 1:50
UniProt:
O15357
Purification:
Protein A
Concentration:
1 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Immunofluorescent analysis of (100% Ice-cold methanol) fixed HeLa cells using anti-SHIP-2 Monoclonal Antibody at dilution of 1:50.
Immunohistochemistry of paraffin-embedded Human cardiac muscle using SHIP-2 Monoclonal Antibody at dilution of 1:100.
Immunohistochemistry of paraffin-embedded Human breast cancer using SHIP-2 Monoclonal Antibody at dilution of 1:100.
Western Blot with SHIP-2 Monoclonal Antibody at dilution of 1:1000. Lane 1: Jurkat (negative control), Lane 2: HeLa, Lane 3: K562 Observed-MW:140 kDa Calculated-MW:139 kDa
