The Recombinant SREBP-1 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C111021) against SREBP-1. This gene encodes a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis. The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC, IF and ELISA applications. It has been validated by IHC analysis of Human lung cancer, Rat spleen. The target is localized to the endoplasmic reticulum membrane, multi-pass membrane protein, Golgi apparatus membrane, multi-pass membrane protein, cytoplasmic vesicle, COPII-coated vesicle membrane, multi-pass membrane protein, at high sterol concentrations, the SCAP-SREBP is retained in the endoplasmic reticulum. Low sterol concentrations promote recruitment into COPII-coated vesicles and transport of the SCAP-SREBP to the Golgi, where it is processed, nucleus, nucleus, nucleus.
Product Name:
Recombinant SREBP-1 Monoclonal Antibody
SKU:
AGEL6327
Size:
50 µL, 100 µL
Antibody Type:
Recombinant Monoclonal Antibody
Synonyms:
BHLHD, BHLHD1, SREBF, SREBF1, SREBP, SREBP1
Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant Human SREBP-1 protein
Applications:
WBIHCIFELISA
Clonality:
Recombinant Monoclonal
Clone No:
C111021
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
SREBP-1
Conjugation:
Unconjugated
Recommended Dilution:
IHC 1:200-1000; WB 1:2000-10000; IF 1:200-1000; ELISA 1:5000-20000
UniProt:
P36956
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Immunohistochemistry of paraffin-embedded human lung cancer using Recombinant SREBP-1 Monoclonal Antibody at dilution of 1:200.
Immunohistochemistry of paraffin-embedded rat spleen using Recombinant SREBP-1 Monoclonal Antibody at dilution of 1:200.
