The Recombinant Stathmin Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C111288) against Stathmin. Involved in the regulation of the microtubule (MT) filament system by destabilizing microtubules. Prevents assembly and promotes disassembly of microtubules. Phosphorylation at Ser-16 may be required for axon formation during neurogenesis. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC, IF and IP applications. It has been validated by WB analysis of Jurkat, Neuro-2a, Mouse brain, IHC analysis of Human lung squamous carcinoma, Human tonsil, Mouse cerebrum, Rat cerebrum, IF analysis of Jurkat and IP analysis of Jurkat cells extracts. The target is localized to the cytoskeleton.
WB 1:500-2000; IHC 1:200-1000; IF 1:50; IP 1:25-50
UniProt:
P16949
Purification:
Protein A
Concentration:
1 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Immunofluorescent analysis of (4% Paraformaldehyde) Jurkat cells using anti-Stathmin Monoclonal Antibody at dilution of 1:50.
Immunohistochemistry of paraffin-embedded Human lung squamous carcinoma using Stathmin Monoclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Human tonsil using Stathmin Monoclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Mouse cerebrum using Stathmin Monoclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Rat cerebrum using Stathmin Monoclonal Antibody at dilutionfixed of 1:1000.
Immunoprecipitation analysis using anti-Stathmin Monoclonal Antibody. Western blot was performed from the immunoprecipitate using Stathmin Monoclonal Antibody at a dilution of 1:50. Lane 1: 10% Input, Lane 2: Stathmin Monoclonal Antibody, Lane 3: Rabbit monoclonal IgG Isotype Observed-MW:18 kDa Calculated-MW:17 kDa
Western Blot with Stathmin Monoclonal Antibody at dilution of 1:2000. Lane 1: Jurkat, Lane 2: Neuro-2a, Lane 3: Mouse brain Observed-MW:18 kDa Calculated-MW:17 kDa
![Anti-Stathmin 1 [R07-5K8] Monoclonal Antibody (AGMB06464)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/277745/733410/anti-stathmin-1-r07-5k8-monoclonal-antibody-agmb06464__88264.1777189896.jpg?c=2 "Anti-Stathmin 1 [R07-5K8] Monoclonal Antibody (AGMB06464)")
