The Recombinant SV2A Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C121611) against SV2A. SV2s are a family of synaptic vesicle proteins expressed in both neurons and endocrine cells. SV2s function in the regulation of synaptic vesicle traffic, cytoplasmic Ca2+ levels in the nerve terminal during repetitive stimulation and the facilitation of synaptic transmission. There are three isoforms of SV2: SV2A, SV2B and SV2C. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB and IHC applications. It has been validated by WB analysis of Mouse brain,?Rat brain and IHC analysis of Human cerebrum, Human pancreas, Human placenta (negative tissue), Mouse cerebrum, Rat cerebrum. The target is localized to the presynapse, cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane.
Product Name:
Recombinant SV2A Monoclonal Antibody
SKU:
AGEL6916
Size:
50 µL, 100 µL
Antibody Type:
Recombinant Monoclonal Antibody
Synonyms:
KIAA, KIAA0736, PSEC, PSEC0174, SV2, SV2A
Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fragment
Applications:
WBIHC
Clonality:
Recombinant Monoclonal
Clone No:
C121611
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
SV2A
Conjugation:
Unconjugated
Observed MW:
100 kDa
Calculated MW:
83 kDa
Recommended Dilution:
WB 1:1000; IHC 1:500-1000
UniProt:
Q7L0J3
Purification:
Protein A
Concentration:
1 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Immunohistochemistry of paraffin-embedded Human cerebrum using SV2A Monoclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Human pancreas using SV2A Monoclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Human placenta (negative tissue) using SV2A Monoclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Mouse cerebrum using SV2A Monoclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Rat cerebrum using SV2A Monoclonal Antibody at dilution of 1:1000.
Western Blot with SV2A Monoclonal Antibody at dilution of 1:1000. Lane 1: Mouse brain, Lane 2: Rat brain Observed-MW:100 kDa Calculated-MW:83 kDa
