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Recombinant Thymidylate Synthase Monoclonal Antibody (RMEA1297)

Datasheet MSDS
SKU RMEA1297
Product Type Antibody
Reactivity Human
Applications WB, IHC, IF, IP
Host Species Rabbit
Clonality Monoclonal
Clone C311297
Conjugate Unconjugated
$606 - $843

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Description

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Recombinant Thymidylate Synthase Monoclonal Antibody

The Recombinant Thymidylate Synthase Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C311297) against Thymidylate Synthase. Thymidylate synthase (TS) is a key enzyme in the synthesis of 2'-deoxythymidine-5'-monophosphate, an essential precursor for DNA biosynthesis. Thymine nucleotide synthase is a key enzyme in the de novo synthesis pathway of mitochondrial thymine. For this reason, it is a key target for cancer chemotherapy, and considered to be the main target of 5-fluorouric acid, 5-fluoro-2'-deoxyribonucleic acid and some folate compounds. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human samples.

This antibody is suitable for WB, IHC, IF and IP applications. It has been validated by WB analysis of HeLa, Jurkat, Raw264.7, 293T, Raji, IHC analysis of Human colon cancer, Human ovarian cancer, IF analysis of Jurkat and IP analysis of Jurkat cells extracts. The target is localized to the nucleus, cytoplasm, mitochondrion, mitochondrion matrix, mitochondrion inner membrane.

Product Name:Recombinant Thymidylate Synthase Monoclonal Antibody
SKU:RMEA1297
Size:50 µL, 100 µL
Antibody Type:Recombinant Monoclonal Antibody
Synonyms:HST, HST422, OK/SW-cl, OK/SW-cl.29, thymidylate synthase, thymidylate synthetase, TMS, TS, TYMS
Reactivity:Human
Immunogen:Recombinant human Thymidylate Synthase fragment
Applications:WBIHCIFIP
Clonality:Recombinant Monoclonal
Clone No:C311297
Host:Rabbit
Isotype:Rabbit IgG
Target:Thymidylate Synthase
Conjugation:Unconjugated
Observed MW:34 kDa
Calculated MW:36 kDa
Recommended Dilution:WB 1:500-1000; IHC 1:100-1000; IF 1:50; IP 1:25-100
UniProt:P04818
Purification:Protein A
Concentration:1 mg/mL
Buffer:PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Product ImageImmunofluorescent analysis of (4% Paraformaldehyde) fixed Jurkat cells using anti-Thymidylate Synthase Monoclonal Antibody at dilution of 1:50.
Product ImageImmunohistochemistry of paraffin-embedded Human colon cancer using Thymidylate Synthase Monoclonal Antibody at dilution of 1:1000.
Product ImageImmunohistochemistry of paraffin-embedded Human ovarian cancer using Thymidylate Synthase Monoclonal Antibody at dilution of 1:100.
Product ImageImmunoprecipitation analysis using anti-Thymidylate Synthase Monoclonal Antibody. Western blot was performed from the immunoprecipitate using Thymidylate Synthase Monoclonal Antibody at a dilution of 1:100. Lane 1: 5% Input, Lane 2: Thymidylate Synthase Monoclonal Antibody, Lane 3: Rabbit monoclonal IgG Isotype Observed-MW:34 kDa Calculated-MW:36 kDa
Product ImageWestern Blot with Thymidylate Synthase Monoclonal Antibody at dilution of 1:1000. Lane 1: HeLa, Lane 2: Jurkat, Lane 3: Raw264.7, Lane 4: 293T, Raji Observed-MW:34 kDa Calculated-MW:36 kDa
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