The Recombinant Thymidylate Synthase Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C171434) against Thymidylate Synthase. Thymidylate synthase is a homodimer composed of two 30 kDa subunits, which catalyze the methylation of deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), an essential step in the formation of thymine nucleotides. Thymidylate synthase is an intracellular enzyme that provides the sole de novo source of thymidylate, making it a required enzyme in DNA biosynthesis with activity highest in proliferating cells. Being the exclusive source of dTMP, investigators have concluded that TS is also an important target for anticancer agents such as 5-fluorouracil (5-FU). 5-FU acts as a TS inhibitor and is active against solid tumors such as colon, breast, head, and neck. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IF and FCM applications. It has been validated by WB analysis of HeLa, Jurkat, HepG2, RAW 264.7, C6, IF analysis of HeLa, Jurkat and FCM analysis of HeLa. The target is localized to the cytoplasm, nucleus.
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Flow cytometric analysis of human Thymidylate Synthase expression on HeLa cells. Cells were stained with purified anti-Human Thymidylate Synthase, then a Alexa Fluor 488- conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of intact cells.
Immunofluorescent analysis of (4% Paraformaldehyde) fixed HeLa cells using anti-Thymidylate Synthase Monoclonal Antibody at dilution of 1:50.
Immunofluorescent analysis of (4% Paraformaldehyde) fixed Jurkat cells using anti-Thymidylate Synthase Monoclonal Antibody at dilution of 1:50.
Western Blot with Thymidylate Synthase Monoclonal Antibody at dilution of 1:2000. Lane 1: HeLa, Lane 2: Jurkat, Lane 3: HepG2, Lane 4: RAW 264.7, Lane 5: C6 Observed-MW:36 kDa Calculated-MW:36 kDa
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