The Recombinant TMS1/ASC Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C311524) against TMS1/ASC. Target of methylation-induced silencing (TMS1)/Apoptosis-associated speck-like protein containing a CARD (ASC), also referred to as PYCARD and CARD5, is a 22-kDa pro-apoptotic protein containing an N-terminal pyrin domain (PYD) and a C-terminal caspase recruitment domain (CARD). The ASC/TMS1 gene was originally found to be aberrantly methylated and silenced in breast cancer cells, and has since been found to be silenced in a number of other cancers, including ovarian cancer, glioblastoma, melanoma, gastric cancer, lung cancer, and prostate cancer. Expression of ASC/TMS1 can be induced by pro-apoptotic/inflammatory stimuli. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human samples.
This antibody is suitable for WB, IHC and IF applications. It has been validated by WB analysis of Jurkat (negative control), THP-1, HL-60, HeLa (negative control), IHC analysis of Human breast cancer, Human colon cancer, Human tonsil and IF analysis of THP-1. The target is localized to the cytoplasm, nucleus, mitochondrion, endoplasmic reticulum, membrane.
Product Name:
Recombinant TMS1/ASC Monoclonal Antibody
SKU:
AGEL6829
Size:
50 µL, 100 µL
Antibody Type:
Recombinant Monoclonal Antibody
Synonyms:
Apoptosis associated speck like protein containing a CARD, Apoptosis-associated speck-like protein containing a CARD, ASC, ASC / TMS, ASC / TMS1, CARD, CARD 5, CARD5, Caspase recruitment domain containing protein 5, Caspase recruitment domain protein 5, Caspase recruitment domain-containing protein 5, hASC, MGC10332, PYCARD, PYD and CARD domain containing, PYD and CARD domain containing protein, PYD and CARD domain-containing protein, Target of methylation induced silencing 1, Target of methylation-induced silencing 1, TMS, TMS 1, TMS-1, TMS1
Reactivity:
Human
Immunogen:
Recombinant human TMS1/ASC fragment
Applications:
WBIHCIF
Clonality:
Recombinant Monoclonal
Clone No:
C311524
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
TMS1/ASC
Conjugation:
Unconjugated
Observed MW:
22, 15 kDa
Calculated MW:
22, 15 kDa
Recommended Dilution:
WB 1:1000; IHC 1:100-500; IF 1:50
UniProt:
Q9ULZ3
Purification:
Protein A
Concentration:
1 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
