The Recombinant TSHB Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C711045) against TSHB. TSHB (Thyroid Stimulating Hormone Subunit Beta) is a Protein Coding gene. Diseases associated with TSHB include Hypothyroidism, Congenital, Nongoitrous, 4 and Central Congenital Hypothyroidism. Among its related pathways are Signaling by GPCR and Peptide hormone metabolism. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human and Rat samples.
This antibody is suitable for WB, IHC, IF and FCM applications. It has been validated by WB analysis of Rat pituitary, IHC analysis of Rat pituitary, IF analysis of HeLa, U-2 OS and FCM analysis of HeLa. The target is localized to the cytoplasm.
WB 1:500-2000; IHC 1:100-500; IF 1:1000-2000; FCM 1:50-100
UniProt:
P01222
Purification:
Protein A
Concentration:
1 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Flow cytometric analysis of human TSHB expression on HeLa cells. Cells were stained with purified anti-Human TSHB, then a Alexa Fluor 488-conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of intact cells.
Immunofluorescent analysis of (100% methanol) fixed HeLa cells using anti-TSHB Monoclonal Antibody at dilution of 1:2000.
Immunofluorescent analysis of (100% methanol) fixed U-2 OS cells using anti-TSHB Monoclonal Antibody at dilution of 1:2000.
Immunohistochemistry of paraffin-embedded Rat pituitary using TSHB Monoclonal Antibody at dilution of 1:500.
Western Blot with TSHB Monoclonal Antibody at dilution of 1:2000. Lane 1: Rat pituitary Observed-MW:16 kDa Calculated-MW:16 kDa
