The Recombinant UCHL1/PGP9.5 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C110077) against UCHL1. The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC-P, ICC/IF, FC and IP applications. It has been validated by WB analysis of A549, SH-SY5Y, 293T, U87-MG, Neuro-2a, Mouse brain, Mouse testis, C6, PC-12, Rat brain, Rat testis and IHC analysis of Human pancreas, Mouse pancreas, Rat pancreas. The target is localized to the cytoplasm, endoplasmic reticulum membrane.
Product Name:
Recombinant UCHL1/PGP9.5 Monoclonal Antibody
SKU:
AGEL5396
Size:
50 µL, 100 µL
Antibody Type:
Recombinant Monoclonal Antibody
Synonyms:
EC:3.4.19.12, Epididymis luminal protein 117, Epididymis secretory protein Li 53, HEL, HEL 117, HEL S 53, HEL-117, HEL-S, HEL-S-53, NDGOA, Neuron Cytoplasmic Protein, Neuron Cytoplasmic Protein 9.5, OTTHUMP00000218137, OTTHUMP00000218139, OTTHUMP00000218140, OTTHUMP00000218141, PARK, Park 5, PARK5, PGP, PGP 9.5, PGP 9.5 (PGP9.5), PGP9.5, PGP95, Protein gene product 9.5, SPG, SPG79, Ubiquitin C terminal esterase L1, Ubiquitin C terminal hydrolase, Ubiquitin C terminal hydrolase L1, Ubiquitin C-terminal hydrolase L1, Ubiquitin carboxyl terminal esterase L1, Ubiquitin carboxyl terminal hydrolase isozyme L1, Ubiquitin carboxyl-terminal hydrolase isozyme L, Ubiquitin carboxyl-terminal hydrolase isozyme L1, Ubiquitin thioesterase L, Ubiquitin thioesterase L1, Ubiquitin thiolesterase, Ubiquitin thiolesterase L1, Uch-L, Uch-L1, UCHL, UCHL-1, UCHL1
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue sections using UCHL1/PGP9.5 Antibody (AGEL5396) at a dilution of 1:300 (10× lens). Heat mediated antigen retrieval was performed with Tris- EDTA buffer (pH 9.0) for 20 min prior to IHC staining.
Immunohistochemical analysis of paraffin-embedded Mouse pancreas tissue sections using UCHL1/PGP9.5 Antibody (AGEL5396) at a dilution of 1:300 (10× lens). Heat mediated antigen retrieval was performed with Tris- EDTA buffer (pH 9.0) for 20 min prior to IHC staining.
Immunohistochemical analysis of paraffin-embedded Rat pancreas tissue sections using UCHL1/PGP9.5 Antibody (AGEL5396) at a dilution of 1:300 (10× lens). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0) for 20 min prior to IHC staining.
Western blot analysis of A549,SH-SY5Y,293T,U87- MG,Neuro-2a,Mouse brain,Mouse testis,C6,PC-12,Rat brain and Rat testis cell lysates using UCHL1/PGP9.5 Antibody (AGEL5396) at a dilution of 1:1500. Observed-MW:25 kDa Calculated-MW:25 kDa