The RFC3 Antibody (CAB4075) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blot applications.RFC3, part of the Replication Factor C complex, plays a crucial role in loading the PCNA protein onto DNA during replication and repair processes. Dysregulation of RFC3 has been linked to genomic instability and cancer development. The RFC3 Polyclonal Antibody binds specifically to RFC3, enabling researchers to detect and analyze its expression in various cell types.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
RFC3 Antibody
SKU:
CAB4075
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
RFC38, RFC3
Positive Sample:
MCF-7, SW480, HL-60, NIH/3T3, 293T, Mouse liver
Cellular Localization:
Nucleus.
Calculated MW:
41kDa
Observed MW:
41kDa
The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kDa. This gene encodes the 38 kDa subunit. This subunit is essential for the interaction between the 140 kDa subunit and the core complex that consists of the 36, 37, and 40 kDa subunits. Alternatively spliced transcript variants encoding distinct isoforms have been described.
Purification Method
Affinity purification
Gene ID
5983
RRID
AB_2765488
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using RFC3 Rabbit pAb (CAB4075) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
