The [KO Validated] RhoC Antibody (CAB1062) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has been extensively validated for use in Western blot applications and is highly specific for detecting RhoC in human samples.RhoC is a member of the Rho GTPase family and is known to regulate the actin cytoskeleton, cell adhesion, and cell motility. Overexpression of RhoC has been linked to increased invasiveness and metastatic potential in various cancer types, making it a promising target for therapeutic intervention.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
[KO Validated] RhoC Antibody
SKU:
CAB1062
Size:
20μL, 100μL
Reactivity:
Human, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Purification Method
Affinity purification
Gene ID
389
RRID
AB_2861495
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from wild type (WT) and RhoC knockout (KO) 293T cells, using [KO Validated] RhoC Rabbit pAb (CAB1062) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 300s.
Western blot analysis of lysates from Rat heart , using [KO Validated] RhoC Rabbit pAb (CAB1062) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
