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Anti-GRK1 Antibody (CAB6497)

SKU:
CAB6497
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction
  • Signal Transduction Antibodies 3 Anti-GRK1 Antibody CAB6497
  • Signal Transduction Antibodies 3 Anti-GRK1 Antibody CAB6497
  • Signal Transduction Antibodies 3 Anti-GRK1 Antibody CAB6497
€139 - €419
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Description

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Antibody Name:Anti-GRK1 Antibody
Antibody SKU:CAB6497
Antibody Size:20uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human GRK1 (NP_002920.1).
Application:WB IHC IF
Recommended Dilution:WB 1:200 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Y79
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human GRK1 (NP_002920.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MDFG SLET VVAN SAFI AARG SFDG SSSQ PSRD KKYL AKLK LPPL SKCE SLRD SLSL EFES VCLE QPIG KKLF QQFL QSAE KHLP ALEL WKDI EDYD TADN DLQP QKAQ TILA QYLD PQAK LFCS FLDE GIVA KFKE GPVE IQDG LFQP LLQA TLAH LGQA
Gene ID:6011
Uniprot:Q15835
Cellular Location:Lipid-anchor, Membrane
Calculated MW:63kDa
Observed MW:65kDa
Synonyms:GRK1, GPRK1, RHOK, RK
Background:This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates rhodopsin and initiates its deactivation. Defects in GRK1 are known to cause Oguchi disease 2 (also known as stationary night blindness Oguchi type-2).
UniProt Protein Function:GRK1: a Ser/Thr of the GRK family. Phosphorylates and inactivates photon-activated rhodopsin. LOF mutations lead to type 2 Oguchi disease, a form of night blindness. A link between RHOK and retinitis pigmentosa is now thought to be unlikely
UniProt Protein Details:

Protein type:Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; EC 2.7.11.14; Kinase, protein; AGC group; GRK family; GRK subfamily

Chromosomal Location of Human Ortholog: 13q34

Molecular Function:G-protein coupled receptor kinase activity; rhodopsin kinase activity; ATP binding; protein kinase activity

Biological Process: rhodopsin mediated signaling; post-embryonic retina morphogenesis in camera-type eye; phototransduction, visible light; regulation of rhodopsin mediated signaling; visual perception; protein amino acid autophosphorylation; photoreceptor cell morphogenesis; regulation of G-protein coupled receptor protein signaling pathway; positive regulation of phosphorylation; negative regulation of apoptosis

Disease: Oguchi Disease 2

NCBI Summary:This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates rhodopsin and initiates its deactivation. Defects in GRK1 are known to cause Oguchi disease 2 (also known as stationary night blindness Oguchi type-2). [provided by RefSeq, Jul 2008]
UniProt Code:Q15835
NCBI GenInfo Identifier:2833269
NCBI Gene ID:6011
NCBI Accession:Q15835.1
UniProt Secondary Accession:Q15835,Q53X14,
UniProt Related Accession:Q15835
Molecular Weight:563
NCBI Full Name:Rhodopsin kinase
NCBI Synonym Full Names:G protein-coupled receptor kinase 1
NCBI Official Symbol:GRK1  
NCBI Official Synonym Symbols:RK; RHOK; GPRK1  
NCBI Protein Information:rhodopsin kinase
UniProt Protein Name:Rhodopsin kinase
UniProt Synonym Protein Names:G protein-coupled receptor kinase 1
UniProt Gene Name:GRK1  
UniProt Entry Name:RK_HUMAN
Anti-GRK1 Antibody (CAB6497)Western blot analysis of extracts of Y79 cells, using GRK1 antibody (CAB6497). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Anti-GRK1 Antibody (CAB6497)Immunofluorescence analysis of rat retina using GRK1 Polyclonal Antibody (CAB6497) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Anti-GRK1 Antibody (CAB6497)Immunofluorescence analysis of mouse retina using GRK1 Polyclonal Antibody (CAB6497) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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