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Anti-RTKN Antibody (CAB6732)

SKU:
CAB6732
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death
  • Cell Death Antibodies 2 Anti-RTKN Antibody CAB6732
  • Cell Death Antibodies 2 Anti-RTKN Antibody CAB6732
€139 - €419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-RTKN Antibody
Antibody SKU:CAB6732
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 254-513 of human RTKN (NP_001015056.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:A-431, HepG2, MCF7
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 254-513 of human RTKN (NP_001015056.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:PLCM TQPT ASGT LRVQ QAGE MQNW AQVH GVLK GTNL FCYR QPED ADTG EEPL LTIA VNKE TRVR AGEL DQAL GRPF TLSI SNQY GDDE VTHT LQTE SREA LQSW MEAL WQLF FDMS QWKQ CCDE IMKI ETPA PRKP PQAL AKQG SLYH EMAI EPLD DIAA VTDI LTQR EGAR LETP PPWL AMFT DQPA LPNP CSPA SVAP APDW THPL PWGR PRTF SLDA VPPD HSPR ARSV APLP PQRS PRTR GLCS KGQP RTWL QSPV
Gene ID:6242
Uniprot:Q9BST9
Cellular Location:
Calculated MW:56kDa/61kDa/62kDa
Observed MW:63kDa
Synonyms:RTKN, rhotekin
Background:This gene encodes a scaffold protein that interacts with GTP-bound Rho proteins. Binding of this protein inhibits the GTPase activity of Rho proteins. This protein may interfere with the conversion of active, GTP-bound Rho to the inactive GDP-bound form by RhoGAP. Rho proteins regulate many important cellular processes, including cytokinesis, transcription, smooth muscle contraction, cell growth and transformation. Dysregulation of the Rho signal transduction pathway has been implicated in many forms of cancer. Alternative splicing results in multiple transcript variants encoding different isoforms.
UniProt Protein Function:RTKN: Mediates Rho signaling to activate NF-kappa-B and may confer increased resistance to apoptosis to cells in gastric tumorigenesis. May play a novel role in the organization of septin structures. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:G protein regulator, misc.

Chromosomal Location of Human Ortholog: 2p13.1

Molecular Function:protein binding; GTP binding; GTP-Rho binding; GTPase inhibitor activity

Biological Process: apoptosis; negative regulation of catalytic activity; signal transduction; Rho protein signal transduction

NCBI Summary:This gene encodes a scaffold protein that interacts with GTP-bound Rho proteins. Binding of this protein inhibits the GTPase activity of Rho proteins. This protein may interfere with the conversion of active, GTP-bound Rho to the inactive GDP-bound form by RhoGAP. Rho proteins regulate many important cellular processes, including cytokinesis, transcription, smooth muscle contraction, cell growth and transformation. Dysregulation of the Rho signal transduction pathway has been implicated in many forms of cancer. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
UniProt Code:Q9BST9
NCBI GenInfo Identifier:74733052
NCBI Gene ID:6242
NCBI Accession:Q9BST9.2
UniProt Secondary Accession:Q9BST9,Q8WVN1, Q96PT6, Q9HB05, H7BXD4,
UniProt Related Accession:Q9BST9
Molecular Weight:563
NCBI Full Name:Rhotekin
NCBI Synonym Full Names:rhotekin
NCBI Official Symbol:RTKN  
NCBI Protein Information:rhotekin; RTKN
UniProt Protein Name:Rhotekin
Protein Family:Rhotekin
UniProt Gene Name:RTKN  
UniProt Entry Name:RTKN_HUMAN
Anti-RTKN Antibody (CAB6732)Western blot analysis of extracts of various cell lines, using RTKN antibody (CAB6732) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-RTKN Antibody (CAB6732)Immunofluorescence analysis of MCF7 cells using RTKN antibody (CAB6732). Blue: DAPI for nuclear staining.

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