Description
Rotavirus A VP6 Recombinant Protein (C-His)
Rotavirus A VP6 Recombinant Protein (C-His) is a recombinant protein expressed in E. coli, spanning Met1-Gln146, with a C-terminal His tag. Predicted molecular weight: 18.29 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Intermediate capsid protein VP6 is a ~44 kDa protein. Intermediate capsid protein that self assembles to form an icosahedral capsid with a T=13 symmetry, which consists of 230 trimers of VP6, with channels at each of its five-fold vertices. For research use only.
This capsid constitutes the middle concentric layer of the viral mature particle. The innermost VP2 capsid and the intermediate VP6 capsid remain intact following cell entry to protect the dsRNA from degradation and to prevent unfavorable antiviral responses in the host cell during all the replication cycle of the virus. Nascent transcripts are transcribed within the structural confines of this double-layered particle (DLP) and are extruded through the channels at the five-fold axes. VP6 is required for the transcription activity of the DLP.
| Product Name: | Rotavirus A VP6 Recombinant Protein (C-His) |
| SKU: | GXRP08802 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | RV-A |
| Synonyms: | Intermediate capsid protein VP6 |
| Tag: | C-His |
| Expression Host: | E. coli |
| Protein Length: | Met1-Gln146 |
| Calculated MW: | 18.29 kDa |
| UniProt: | P03530 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
