Anti-RPL11 Antibody (CAB6407)

Antibody Name:	Anti-RPL11 Antibody
Antibody SKU:	CAB6407
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-177 of human RPL11 (NP_001186731.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, Jurkat, 293T, MCF7, Mouse liver, Mouse spleen, Mouse pancreas, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-177 of human RPL11 (NP_001186731.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MADQ GEKE NPMR ELRI RKLC LNIC VGES GDRL TRAA KVLE QLTG QTPV FSKA RYTV RSFG IRRN EKIA VHCT VRGA KAEE ILEK GLKV REYE LRKN NFSD TGNF GFGI QEHI DLGI KYDP SIGI YGLD FYVV LGRP GFSI ADKK RRTG CIGA KHRI SKEE AMRW FQQK YDGI ILPG K
Gene ID:	6135
Uniprot:	P62913
Cellular Location:	Nucleus, nucleolus
Calculated MW:	20kDa
Observed MW:	20kDa

Synonyms:

RPL11, DBA7, GIG34, L11

Background:

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L5P family of ribosomal proteins. It is located in the cytoplasm. The protein probably associates with the 5S rRNA. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

UniProt Protein Function:	Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. As part of the 5S RNP/5S ribonucleoprotein particle it is an essential component of the LSU, required for its formation and the maturation of rRNAs (PubMed:19061985, PubMed:12962325, PubMed:24120868). It also couples ribosome biogenesis to p53/TP53 activation. As part of the 5S RNP it accumulates in the nucleoplasm and inhibits MDM2, when ribosome biogenesis is perturbed, mediating the stabilization and the activation of TP53 (PubMed:24120868). Promotes nucleolar location of PML.
NCBI Summary:	Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L5P family of ribosomal proteins. It is located in the cytoplasm. The protein probably associates with the 5S rRNA. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Dec 2010]
UniProt Code:	P62913
NCBI GenInfo Identifier:	51702795
NCBI Gene ID:	6135
NCBI Accession:	P62913.2
UniProt Secondary Accession:	P62913,P25121, P39026, Q8TDH2, Q9Y674,
UniProt Related Accession:	P62913
Molecular Weight:	20 kDa
NCBI Full Name:	60S ribosomal protein L11
NCBI Synonym Full Names:	ribosomal protein L11
NCBI Official Symbol:	RPL11
NCBI Official Synonym Symbols:	L11; uL5; DBA7; GIG34
NCBI Protein Information:	60S ribosomal protein L11
UniProt Protein Name:	60S ribosomal protein L11
UniProt Synonym Protein Names:	CLL-associated antigen KW-12; Large ribosomal subunit protein uL5
Protein Family:	50S ribosomal protein
UniProt Gene Name:	RPL11

	Western blot analysis of extracts of various cell lines, using RPL11 antibody (CAB6407) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of L929 cells using RPL11 antibody (CAB6407) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

Anti-RPL11 Antibody (CAB13542)

RPL11 Antibody (PACO04941)

RPL11 Antibody (PACO11907)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)