Anti-RPL24 Antibody (CAB14255)

Antibody Name:	Anti-RPL24 Antibody
Antibody SKU:	CAB14255
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 38-157 of human RPL24 (NP_000977.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HT-29, HepG2, HeLa

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 38-157 of human RPL24 (NP_000977.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SAFL SKRN PRQI NWTV LYRR KHKK GQSE EIQK KRTR RAVK FQRA ITGA SLAD IMAK RNQK PEVR KAQR EQAI RAAK EAKK AKQA SKKT AMAA AKAP TKAA PKQK IVKP VKVS APRV GGKR
Gene ID:	6152
Uniprot:	P83731
Cellular Location:
Calculated MW:	17kDa
Observed MW:	18kDa

Synonyms:

RPL24, HEL-S-310, L24

Background:

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L24E family of ribosomal proteins. It is located in the cytoplasm. This gene has been referred to as ribosomal protein L30 because the encoded protein shares amino acid identity with the L30 ribosomal proteins from S. cerevisiae; however, its official name is ribosomal protein L24. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

UniProt Protein Function:	RPL24: a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L24E family of ribosomal proteins. It is located in the cytoplasm. This gene has been referred to as ribosomal protein L30 because the encoded protein shares amino acid identity with the L30 ribosomal proteins from S. cerevisiae; however, its official name is ribosomal protein L24. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
UniProt Protein Details:	Protein type:Translation; Ribosomal Chromosomal Location of Human Ortholog: 3q12 Cellular Component: membrane; cytosol Molecular Function:structural constituent of ribosome; RNA binding Biological Process: SRP-dependent cotranslational protein targeting to membrane; translational elongation; cellular protein metabolic process; viral reproduction; translation; translational initiation; mRNA catabolic process, nonsense-mediated decay; viral transcription; gene expression; translational termination; viral infectious cycle
NCBI Summary:	Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L24E family of ribosomal proteins. It is located in the cytoplasm. This gene has been referred to as ribosomal protein L30 because the encoded protein shares amino acid identity with the L30 ribosomal proteins from S. cerevisiae; however, its official name is ribosomal protein L24. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
UniProt Code:	P83731
NCBI GenInfo Identifier:	41393532
NCBI Gene ID:	6152
NCBI Accession:	P83731.1
UniProt Related Accession:	P83731
Molecular Weight:
NCBI Full Name:	60S ribosomal protein L24
NCBI Synonym Full Names:	ribosomal protein L24
NCBI Official Symbol:	RPL24
NCBI Official Synonym Symbols:	L24; HEL-S-310
NCBI Protein Information:	60S ribosomal protein L24
UniProt Protein Name:	60S ribosomal protein L24
UniProt Synonym Protein Names:	60S ribosomal protein L30
UniProt Gene Name:	RPL24
UniProt Entry Name:	RL24_HUMAN

	Western blot analysis of extracts of various cell lines, using RPL24 antibody (CAB14255) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded human thyroid cancer using RPL24 antibody (CAB14255) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon carcinoma using RPL24 antibody (CAB14255) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of L929 cells using RPL24 Polyclonal Antibody (CAB14255) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies

RPL24 Antibody (PACO11918)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)