Anti-RPL32 Antibody (CAB13001)

Antibody Name:	Anti-RPL32 Antibody
Antibody SKU:	CAB13001
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-135 of human RPL32 (NP_000985.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, HepG2, NIH/3T3, Mouse liver, Mouse stomach, Mouse spleen, Rat ovary

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-135 of human RPL32 (NP_000985.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAAL RPLV KPKI VKKR TKKF IRHQ SDRY VKIK RNWR KPRG IDNR VRRR FKGQ ILMP NIGY GSNK KTKH MLPS GFRK FLVH NVKE LEVL LMCN KSYC AEIA HNVS SKNR KAIV ERAA QLAI RVTN PNAR LRSE ENE
Gene ID:	6161
Uniprot:	P62910
Cellular Location:
Calculated MW:	15kDa
Observed MW:	16kDa

Synonyms:

RPL32, L32, PP9932

Background:

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L32E family of ribosomal proteins. It is located in the cytoplasm. Although some studies have mapped this gene to 3q13.3-q21, it is believed to map to 3p25-p24. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternatively spliced transcript variants encoding the same protein have been observed for this gene.

NCBI Summary:	Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L32E family of ribosomal proteins. It is located in the cytoplasm. Although some studies have mapped this gene to 3q13.3-q21, it is believed to map to 3p25-p24. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternatively spliced transcript variants encoding the same protein have been observed for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	P62910
NCBI GenInfo Identifier:	4506635
NCBI Gene ID:	6161
NCBI Accession:	NP_000985
UniProt Related Accession:	P62910
Molecular Weight:	15kDa
NCBI Full Name:	60S ribosomal protein L32
NCBI Synonym Full Names:	ribosomal protein L32
NCBI Official Symbol:	RPL32
NCBI Official Synonym Symbols:	L32; PP9932
NCBI Protein Information:	60S ribosomal protein L32
UniProt Protein Name:	60S ribosomal protein L32
Protein Family:	50S ribosomal protein
UniProt Gene Name:	RPL32
UniProt Entry Name:	RL32_HUMAN

	Western blot analysis of extracts of various cell lines, using RPL32 antibody (CAB13001) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded rat brain using RPL32 antibody (CAB13001) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human lung cancer using RPL32 antibody (CAB13001) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse spinal cord using RPL32 antibody (CAB13001) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of L929 cells using RPL32 Polyclonal Antibody (CAB13001) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies

RPL32 Antibody (PACO11925)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)