Anti-RPL34 Antibody (CAB15716)

Antibody Name:	Anti-RPL34 Antibody
Antibody SKU:	CAB15716
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-117 of human RPL34 (NP_000986.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	Mouse brain, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-117 of human RPL34 (NP_000986.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MVQR LTYR RRLS YNTA SNKT RLSR TPGN RIVY LYTK KVGK APKS ACGV CPGR LRGV RAVR PKVL MRLS KTKK HVSR AYGG SMCA KCVR DRIK RAFL IEEQ KIVV KVLK AQAQ SQKA K
Gene ID:	6164
Uniprot:	P49207
Cellular Location:
Calculated MW:	13kDa
Observed MW:	13kDa

Synonyms:

RPL34, L34

Background:

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L34E family of ribosomal proteins. It is located in the cytoplasm. This gene originally was thought to be located at 17q21, but it has been mapped to 4q. Overexpression of this gene has been observed in some cancer cells. Alternative splicing results in multiple transcript variants, all encoding the same isoform. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

UniProt Protein Function:	RPL34: a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L34E family of ribosomal proteins. It is located in the cytoplasm. This gene originally was thought to be located at 17q21, but it has been mapped to 4q. Transcript variants derived from alternative splicing, alternative transcription initiation sites, and/or alternative polyadenylation exist; these variants encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
UniProt Protein Details:	Protein type:Translation; Ribosomal Chromosomal Location of Human Ortholog: 4q25 Cellular Component: cytoplasm; cytosol; nucleolus Molecular Function:RNA binding Biological Process: mRNA catabolic process, nonsense-mediated decay; ribosome biogenesis and assembly; rRNA processing; SRP-dependent cotranslational protein targeting to membrane; translational initiation; viral transcription
NCBI Summary:	Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L34E family of ribosomal proteins. It is located in the cytoplasm. This gene originally was thought to be located at 17q21, but it has been mapped to 4q. Overexpression of this gene has been observed in some cancer cells. Alternative splicing results in multiple transcript variants, all encoding the same isoform. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Feb 2016]
UniProt Code:	P49207
NCBI GenInfo Identifier:	22002070
NCBI Gene ID:	6164
NCBI Accession:	P49207.3
UniProt Secondary Accession:	P49207,Q6FG66, Q9BUZ2,
UniProt Related Accession:	P49207
Molecular Weight:	13,293 Da
NCBI Full Name:	60S ribosomal protein L34
NCBI Synonym Full Names:	ribosomal protein L34
NCBI Official Symbol:	RPL34
NCBI Official Synonym Symbols:	L34
NCBI Protein Information:	60S ribosomal protein L34
UniProt Protein Name:	60S ribosomal protein L34
Protein Family:	50S ribosomal protein
UniProt Gene Name:	RPL34
UniProt Entry Name:	RL34_HUMAN

	Western blot analysis of extracts of various cell lines, using RPL34 antibody (CAB15716) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 3min.
	Immunofluorescence analysis of U-2 OS cells using RPL34 antibody (CAB15716) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

RPL34 Antibody (PACO01443)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)