Anti-RPLP1 Antibody (CAB6725)

Antibody Name:	Anti-RPLP1 Antibody
Antibody SKU:	CAB6725
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat, Zebrafish
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-114 of human RPLP1 (NP_000994.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat, Zebrafish
Positive Samples:	SW480, Mouse spleen, Mouse lung, Mouse brain, Rat spleen, Rat lung, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-114 of human RPLP1 (NP_000994.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MASV SELA CIYS ALIL HDDE VTVT EDKI NALI KAAG VNVE PFWP GLFA KALA NVNI GSLI CNVG AGGP APAA GAAP AGGP APST AAAP AEEK KVEA KKEE SEES DDDM GFGL FD
Gene ID:	6176
Uniprot:	P05386
Cellular Location:
Calculated MW:	8kDa/11kDa
Observed MW:	17kDa

Synonyms:

RPLP1, LP1, P1, RPP1

Background:

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal phosphoprotein that is a component of the 60S subunit. The protein, which is a functional equivalent of the E. coli L7/L12 ribosomal protein, belongs to the L12P family of ribosomal proteins. It plays an important role in the elongation step of protein synthesis. Unlike most ribosomal proteins, which are basic, the encoded protein is acidic. Its C-terminal end is nearly identical to the C-terminal ends of the ribosomal phosphoproteins P0 and P2. The P1 protein can interact with P0 and P2 to form a pentameric complex consisting of P1 and P2 dimers, and a P0 monomer. The protein is located in the cytoplasm. Two alternatively spliced transcript variants that encode different proteins have been observed. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

UniProt Protein Function:	RPLP1: Plays an important role in the elongation step of protein synthesis. Belongs to the ribosomal protein L12P family.
UniProt Protein Details:	Protein type:Translation; Ribosomal Chromosomal Location of Human Ortholog: 15q22 Cellular Component: cytoplasm; cytosol; focal adhesion Molecular Function:protein binding; protein kinase activator activity; structural constituent of ribosome Biological Process: mRNA catabolic process, nonsense-mediated decay; positive regulation of protein kinase activity; rRNA processing; SRP-dependent cotranslational protein targeting to membrane; translational initiation; viral transcription
NCBI Summary:	Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal phosphoprotein that is a component of the 60S subunit. The protein, which is a functional equivalent of the E. coli L7/L12 ribosomal protein, belongs to the L12P family of ribosomal proteins. It plays an important role in the elongation step of protein synthesis. Unlike most ribosomal proteins, which are basic, the encoded protein is acidic. Its C-terminal end is nearly identical to the C-terminal ends of the ribosomal phosphoproteins P0 and P2. The P1 protein can interact with P0 and P2 to form a pentameric complex consisting of P1 and P2 dimers, and a P0 monomer. The protein is located in the cytoplasm. Two alternatively spliced transcript variants that encode different proteins have been observed. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
UniProt Code:	P05386
NCBI GenInfo Identifier:	133051
NCBI Gene ID:	6176
NCBI Accession:	P05386.1
UniProt Secondary Accession:	P05386,A6NIB2,
UniProt Related Accession:	P05386
Molecular Weight:	8,804 Da
NCBI Full Name:	60S acidic ribosomal protein P1
NCBI Synonym Full Names:	ribosomal protein lateral stalk subunit P1
NCBI Official Symbol:	RPLP1
NCBI Official Synonym Symbols:	P1; LP1; RPP1
NCBI Protein Information:	60S acidic ribosomal protein P1
UniProt Protein Name:	60S acidic ribosomal protein P1
UniProt Gene Name:	RPLP1
UniProt Entry Name:	RLA1_HUMAN

	Western blot analysis of extracts of various cell lines, using RPLP1 antibody (CAB6725) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded human stomach using RPLP1 antibody (CAB6725) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat brain using RPLP1 antibody (CAB6725) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using RPLP1 antibody (CAB6725) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of MCF7 cells using RPLP1 antibody (CAB6725). Blue: DAPI for nuclear staining.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)