Anti-RPS5 Antibody (CAB6975)

Antibody Name:	Anti-RPS5 Antibody
Antibody SKU:	CAB6975
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-204 of human RPS5 (NP_001000.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	MCF7, HL-60, HeLa, HepG2, Jurkat, Mouse spleen, Mouse lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-204 of human RPS5 (NP_001000.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MTEW ETAA PAVA ETPD IKLF GKWS TDDV QIND ISLQ DYIA VKEK YAKY LPHS AGRY AAKR FRKA QCPI VERL TNSM MMHG RNNG KKLM TVRI VKHA FEII HLLT GENP LQVL VNAI INSG PRED STRI GRAG TVRR QAVD VSPL RRVN QAIW LLCT GARE AAFR NIKT IAEC LADE LINA AKGS SNSY AIKK KDEL ERVA KSNR
Gene ID:	6193
Uniprot:	P46782
Cellular Location:
Calculated MW:	22kDa
Observed MW:	23kDa

Synonyms:

RPS5, S5

Background:

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S7P family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

UniProt Protein Function:	RPS5: a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S7P family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
UniProt Protein Details:	Protein type:RNA-binding; Ribosomal; Translation Chromosomal Location of Human Ortholog: 19q13.4 Cellular Component: focal adhesion; membrane; ribonucleoprotein complex; cytosol Molecular Function:mRNA binding; structural constituent of ribosome; RNA binding Biological Process: SRP-dependent cotranslational protein targeting to membrane; translational elongation; cellular protein metabolic process; translation; viral reproduction; regulation of translational fidelity; mRNA catabolic process, nonsense-mediated decay; translational initiation; viral transcription; gene expression; viral infectious cycle; translational termination
NCBI Summary:	Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S7P family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
UniProt Code:	P46782
NCBI GenInfo Identifier:	22002064
NCBI Gene ID:	6193
NCBI Accession:	P46782.4
UniProt Secondary Accession:	P46782,Q96BN0, B2R4T2,
UniProt Related Accession:	P46782
Molecular Weight:	204
NCBI Full Name:	40S ribosomal protein S5
NCBI Synonym Full Names:	ribosomal protein S5
NCBI Official Symbol:	RPS5
NCBI Official Synonym Symbols:	S5
NCBI Protein Information:	40S ribosomal protein S5
UniProt Protein Name:	40S ribosomal protein S5
Protein Family:	30S ribosomal protein
UniProt Gene Name:	RPS5
UniProt Entry Name:	RS5_HUMAN

	Western blot analysis of extracts of various cell lines, using RPS5 antibody (CAB6975) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of MCF7 cells using RPS5 antibody (CAB6975). Blue: DAPI for nuclear staining.

Antibodies

RPS5 Antibody (PACO04948)

RPS5 Antibody (PACO11958)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)