Anti-RPS6 Antibody (CAB6058)

Antibody Name:	Anti-RPS6 Antibody
Antibody SKU:	CAB6058
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RPS6 (NP_001001.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:1000 IHC 1:50 - 1:100 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	BxPC-3, A-431, NIH/3T3, Mouse thymus, Rat spleen, Rat lung

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RPS6 (NP_001001.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MKLN ISFP ATGC QKLI EVDD ERKL RTFY EKRM ATEV AADA LGEE WKGY VVRI SGGN DKQG FPMK QGVL THGR VRLL LSKG HSCY RPRR TGER KRKS VRGC
Gene ID:	6194
Uniprot:	P62753
Cellular Location:
Calculated MW:	28kDa
Observed MW:	28kDa

Synonyms:

RPS6, S6, RPS6

Background:

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

UniProt Protein Function:	S6: May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA. Belongs to the ribosomal protein S6e family.
UniProt Protein Details:	Protein type:Translation Chromosomal Location of Human Ortholog: 9p21 Cellular Component: polysome; small ribosomal subunit; membrane; perinuclear region of cytoplasm; dendrite; cytoplasm; nucleolus; nucleus; ribonucleoprotein complex; cytosol Molecular Function:protein binding; structural constituent of ribosome; protein kinase binding Biological Process: SRP-dependent cotranslational protein targeting to membrane; erythrocyte development; TOR signaling pathway; viral reproduction; translation; positive regulation of apoptosis; T cell differentiation in the thymus; gastrulation; translational termination; viral infectious cycle; glucose homeostasis; oogenesis stage; T cell proliferation during immune response; translational initiation; ribosomal small subunit assembly and maintenance; viral transcription; activated T cell apoptosis; placenta development; mitosis; mitotic cell cycle checkpoint; ribosomal small subunit biogenesis and assembly; translational elongation; cellular protein metabolic process; insulin receptor signaling pathway; mRNA catabolic process, nonsense-mediated decay; gene expression; rRNA processing; G1/S transition of mitotic cell cycle; negative regulation of apoptosis
NCBI Summary:	Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
UniProt Code:	P62753
NCBI GenInfo Identifier:	51338632
NCBI Gene ID:	6194
NCBI Accession:	P62753.1
UniProt Secondary Accession:	P62753,P08227, P10660, Q4VBY7, Q8N6Z7,
UniProt Related Accession:	P62753
Molecular Weight:	249
NCBI Full Name:	40S ribosomal protein S6
NCBI Synonym Full Names:	ribosomal protein S6
NCBI Official Symbol:	RPS6
NCBI Official Synonym Symbols:	S6
NCBI Protein Information:	40S ribosomal protein S6; phosphoprotein NP33
UniProt Protein Name:	40S ribosomal protein S6
UniProt Synonym Protein Names:	Phosphoprotein NP33
Protein Family:	30S ribosomal protein
UniProt Gene Name:	RPS6
UniProt Entry Name:	RS6_HUMAN

	Western blot analysis of extracts of various cell lines, using RPS6 antibody (CAB6058) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min.
	Western blot analysis of extracts of various cell lines, using RPS6 antibody (CAB6058) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat brain using RPS6 Rabbit pAb (CAB6058) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of C6 cells using RPS6 Rabbit pAb (CAB6058) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH-3T3 cells using RPS6 Rabbit pAb (CAB6058) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-Phospho-RPS6-S235 Antibody (CABP0227)	S6 Ribosomal Protein (Phospho-Ser240) Colorimetric Cell-Based ELISA Kit
Anti-Phospho-RPS6-S240/244 Antibody (CABP0537)	S6 Ribosomal Protein (Phospho-Ser235) Colorimetric Cell-Based ELISA Kit
Anti-Phospho-RPS6-S235/236 Antibody (CABP0538)
Phospho-RPS6 (S235) Antibody (PACO04949)
Phospho-RPS6 (S235/S236) Antibody (PACO04950)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)