The p70 S6 Kinase 1 Antibody (CAB16658) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly reactive with human samples and has been validated for use in Western blot applications. It binds specifically to the RPS6KB1 protein, allowing for accurate detection and analysis in a variety of cell types.RPS6KB1, also known as p70 S6 kinase, is a key player in the mTOR signaling pathway, which regulates cell growth, survival, and metabolism. Dysregulation of RPS6KB1 has been associated with various diseases, including cancer, diabetes, and neurological disorders.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
p70 S6 Kinase 1 Antibody
SKU:
CAB16658
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17.
Purification Method
Affinity purification
Gene ID
6198
RRID
AB_2772104
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using p70 S6 Kinase 1 Rabbit pAb (CAB16658) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Western blot analysis of various lysates using p70 S6 Kinase 1 Rabbit pAb (CAB16658) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using p70 S6 Kinase 1 1 Rabbit pAb (CAB16658) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse lung using p70 S6 Kinase 1 1 Rabbit pAb (CAB16658) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of NIH/3T3 cells using p70 S6 Kinase 1 Rabbit pAb (CAB16658) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
