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Anti-RRM1 Antibody (CAB1152)

SKU:
CAB1152
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
  • Epigenetics and Nuclear Signaling Antibodies 1 Anti-RRM1 Antibody CAB1152
  • Epigenetics and Nuclear Signaling Antibodies 1 Anti-RRM1 Antibody CAB1152
  • Epigenetics and Nuclear Signaling Antibodies 1 Anti-RRM1 Antibody CAB1152
€139 - €419
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Description

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Antibody Name:Anti-RRM1 Antibody
Antibody SKU:CAB1152
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 593-792 of human RRM1 (NP_001024.1).
Application:WB IHC IF IP
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, MCF7, HT-29, A-549, SW620, Mouse testis, Mouse spleen, Mouse thymus
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 593-792 of human RRM1 (NP_001024.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:IRNS LLIA PMPT ASTA QILG NNES IEPY TSNI YTRR VLSG EFQI VNPH LLKD LTER GLWH EEMK NQII ACNG SIQS IPEI PDDL KQLY KTVW EISQ KTVL KMAA ERGA FIDQ SQSL NIHI AEPN YGKL TSMH FYGW KQGL KTGM YYLR TRPA ANPI QFTL NKEK LKDK EKVS KEEE EKER NTAA MVCS LENR DECL MCGS
Gene ID:6240
Uniprot:P23921
Cellular Location:Cytoplasm
Calculated MW:90kDa
Observed MW:90kDa
Synonyms:RRM1, R1, RIR1, RR1
Background:This gene encodes the large and catalytic subunit of ribonucleotide reductase, an enzyme essential for the conversion of ribonucleotides into deoxyribonucleotides. A pool of available deoxyribonucleotides is important for DNA replication during S phase of the cell cycle as well as multiple DNA repair processes. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:RRM1: an enzyme involved in DNA replication that provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Belongs to the ribonucleoside diphosphate reductase large chain family. Heterodimer of a large and a small subunit. Heterodimer with small subunit RRM2 or RRM2B. The heterodimer with RRM2 has higher catalytic activity than the heterodimer with RRM2B. Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding to separate specificity and activation sites on the M1 subunit. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction. Stimulated by ATP and inhibited by dATP binding to the activity site. Two distinct regulatory sites have been defined: the specificity site, which controls substrate specificity, and the activity site which regulates overall catalytic activity. A substrate-binding catalytic site, located on M1, is formed only in the presence of the second subunit M2. The level of the enzyme activity is closely correlated with the growth rate of a cell and appears to vary with the cell cycle. Patients with advanced non-small cell lung cancer responded favorably to gemcitabine.
UniProt Protein Details:

Protein type:Nucleotide Metabolism - purine; Other Amino Acids Metabolism - glutathione; DNA replication; EC 1.17.4.1; Nucleotide Metabolism - pyrimidine; Oxidoreductase

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: nucleoplasm; cell projection; cell soma; cytoplasm; nuclear envelope; cytosol

Molecular Function:protein binding; ATP binding; ribonucleoside-diphosphate reductase activity

Biological Process: pyrimidine base metabolic process; nucleobase, nucleoside and nucleotide metabolic process; nucleobase, nucleoside and nucleotide interconversion; retina development in camera-type eye; cell proliferation in forebrain; male gonad development; deoxyribonucleotide biosynthetic process; protein heterotetramerization; mitotic cell cycle; response to ionizing radiation; DNA replication

NCBI Summary:This gene encodes the large and catalytic subunit of ribonucleotide reductase, an enzyme essential for the conversion of ribonucleotides into deoxyribonucleotides. A pool of available deoxyribonucleotides is important for DNA replication during S phase of the cell cycle as well as multiple DNA repair processes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]
UniProt Code:P23921
NCBI GenInfo Identifier:132608
NCBI Gene ID:6240
NCBI Accession:P23921.1
UniProt Secondary Accession:P23921,P07742,
UniProt Related Accession:P23921
Molecular Weight:90kDa
NCBI Full Name:Ribonucleoside-diphosphate reductase large subunit
NCBI Synonym Full Names:ribonucleotide reductase catalytic subunit M1
NCBI Official Symbol:RRM1  
NCBI Official Synonym Symbols:R1; RR1; RIR1  
NCBI Protein Information:ribonucleoside-diphosphate reductase large subunit
UniProt Protein Name:Ribonucleoside-diphosphate reductase large subunit
UniProt Synonym Protein Names:Ribonucleoside-diphosphate reductase subunit M1; Ribonucleotide reductase large subunit
UniProt Gene Name:RRM1  
UniProt Entry Name:RIR1_HUMAN
Anti-RRM1 Antibody (CAB1152)Western blot analysis of extracts of various cell lines, using RRM1 antibody (CAB1152) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-RRM1 Antibody (CAB1152)Immunohistochemistry of paraffin-embedded human colon using RRM1 Rabbit pAb (CAB1152) at dilution of 1:100 (40x lens).
Anti-RRM1 Antibody (CAB1152)Immunofluorescence analysis of HeLa cells using RRM1 Rabbit pAb (CAB1152) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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