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Cell Biology Antibodies 15 Anti-RUNX1/2/3 Antibody CAB5115
Key Features

RUNX1/2/3 Rabbit Monoclonal Antibody (CAB5115)

Datasheet MSDS
SKU CAB5115
Product Type Antibody
Antibody Type Monoclonal Antibody
Reactivity Human, Mouse
Host Species Rabbit
Synonyms AML1, AML1-EVI-1, AMLCR1, CBF2alpha, CBFA2, EVI-1, PEBP2aB, PEBP2alpha, RUNX1/2/3
Research Use Cell Biology
$173 - $603

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Description

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RUNX1/2/3 Monoclonal Antibody (CAB5115)

The RUNX1/2/3 Monoclonal Antibody (CAB5115) is a high-quality antibody developed for reliable detection and analysis of target proteins. These proteins are key regulators of cell differentiation and development, with important roles in hematopoiesis, osteogenesis, and neurogenesis.Raised in rabbits, this antibody offers high specificity and sensitivity in detecting Runx1, Runx2, and Runx3 in various sample types, including human tissues and cell lines. It has been validated for use in applications such as immunofluorescence, immunohistochemistry, and Western blotting, providing reliable results for your experiments.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.

Product Name:RUNX1/2/3 Monoclonal Antibody
SKU:CAB5115
Size:20μL, 100μL
Reactivity:Human, Mouse
Clone Number:ARC1162
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:LPPP YPGS SQAQ GGPF QASS PSYH LYYG ASAG SYQF SMVG GERS PPRI LPPC TNAS TGSA LLNP SLPN QSDV VEAE GSHS NSPT NMAP SARL EEAV WRPY
Tested Applications:WB ELISA
Recommended Dilution:
WB1:500 - 1:1000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:AML1, AML1-EVI-1, AMLCR1, CBF2alpha, CBFA2, EVI-1, PEBP2aB, PEBP2alpha, RUNX1/2/3
Positive Sample:HeLa, 293T, Mouse lung
Cellular Localization:Nucleus.
Calculated MW:51kDa
Observed MW:45-55kDa

Core binding factor (CBF) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters. The protein encoded by this gene represents the alpha subunit of CBF and is thought to be involved in the development of normal hematopoiesis. Chromosomal translocations involving this gene are well-documented and have been associated with several types of leukemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Purification MethodAffinity purification
Gene ID861 864 860
RRIDAB_2863452
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using RUNX1/2/3 Rabbit mAb (CAB5115) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
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