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Anti-RUSC1 Antibody (CAB15417)

SKU:
CAB15417
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction
  • Signal Transduction Antibodies 2 Anti-RUSC1 Antibody CAB15417
  • Signal Transduction Antibodies 2 Anti-RUSC1 Antibody CAB15417
  • Signal Transduction Antibodies 2 Anti-RUSC1 Antibody CAB15417
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-RUSC1 Antibody
Antibody SKU:CAB15417
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-320 of human RUSC1 (NP_055143.2).
Application:WB IF
Recommended Dilution:WB 1:200 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:A-549, Mouse brain, Mouse lung, Rat lung
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-320 of human RUSC1 (NP_055143.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAEA QSGT GQLQ EQKK GLLI AVSV SVDK IISH FGAA RNLV QKAQ LGDS RLSP DVGH LVLT TLCP ALHA LVAD GLKP FRKD LITG QRRS SPWS VVEA SVKP GSST RSLG TLYS QVSR LAPL SSSR SRFH AFIL GLLN TKQL ELWF SSLQ EDAG LLSL LYLP TGFF SLAR GGCP SLST ELLL LLQP LSVL TFHL DLLF EHHH HLPL GPPQ APAP PGPP PALQ QTMQ AMLH FGGR LAQS LRGT SKEA ASDP SDSP NLPT PGSW WEQL TQAS RVYA SGGT EGFP LSRW APGR HGTA AEEG AQER PLPT DEMA PGRG LWLG RLFG
Gene ID:23623
Uniprot:Q9BVN2
Cellular Location:Cell junction, Cytoplasm, Cytoplasmic vesicle, Early endosome, Golgi apparatus, Nucleus, cytoskeleton, postsynaptic cell membrane, postsynaptic density, synapse
Calculated MW:46kDa/52kDa/85kDa/96kDa
Observed MW:55/100kDa
Synonyms:RUSC1, NESCA
Background:
UniProt Protein Function:RUSC1: Putative signaling adapter which may play a role in neuronal differentiation. May be involved in regulation of NGF- dependent neurite outgrowth. Proposed to play a role in neuronal vesicular trafficking, specifically involving pre-synaptic membrane proteins. Seems to be involved in signaling pathways that are regulated by the prolonged activation of MAPK. Can regulate the polyubiquitination of IKBKG and thus may be involved in regulation of the NF-kappa-B pathway. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Adaptor/scaffold

Chromosomal Location of Human Ortholog: 1q21-q22

Cellular Component: microtubule cytoskeleton; Golgi apparatus; postsynaptic membrane; microtubule; postsynaptic density; cytoplasmic membrane-bound vesicle; early endosome; cytoplasm; cytoplasmic vesicle; cell junction; nucleus

Molecular Function:protein binding; SH3/SH2 adaptor activity; actin binding

Biological Process: protein polyubiquitination; positive regulation of signal transduction

UniProt Code:Q9BVN2
NCBI GenInfo Identifier:157412245
NCBI Gene ID:23623
NCBI Accession:NP_001098673
UniProt Related Accession:Q9BVN2
Molecular Weight:55; 100kDa
NCBI Full Name:RUN and SH3 domain-containing protein 1 isoform a
NCBI Synonym Full Names:RUN and SH3 domain containing 1
NCBI Official Symbol:RUSC1  
NCBI Official Synonym Symbols:NESCA  
NCBI Protein Information:RUN and SH3 domain-containing protein 1
UniProt Protein Name:RUN and SH3 domain-containing protein 1
UniProt Synonym Protein Names:New molecule containing SH3 at the carboxy-terminus; Nesca
Protein Family:RUN and SH3 domain-containing protein
UniProt Gene Name:RUSC1  
UniProt Entry Name:RUSC1_HUMAN
Anti-RUSC1 Antibody (CAB15417)Western blot analysis of extracts of various cell lines, using RUSC1 antibody (CAB15417) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-RUSC1 Antibody (CAB15417)Immunofluorescence analysis of mouse brain using RUSC1 antibody (CAB15417) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-RUSC1 Antibody (CAB15417)Immunofluorescence analysis of mouse brain using RUSC1 antibody (CAB15417) at dilution of 1:100. Blue: DAPI for nuclear staining.

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