Anti-RUVBL1 Antibody (CAB5723)

Antibody Name:	Anti-RUVBL1 Antibody
Antibody SKU:	CAB5723
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human RUVBL1 (NP_003698.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:20 - 1:50
Reactivity:	Human, Mouse
Positive Samples:	HL-60, MCF7, HepG2, 293T, Mouse spleen, Mouse skeletal muscle, Mouse thymus, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human RUVBL1 (NP_003698.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MKIE EVKS TTKT QRIA SHSH VKGL GLDE SGLA KQAA SGLV GQEN AREA CGVI VELI KSKK MAGR AVLL AGPP GTGK TALA LAIA QELG SKVP FCPM VGSE VYST EIKK TEVL MENF RRAI GLRI KETK EVYE GEVT ELTP CETE NPMG GYGK TISH VIIG LKTA KGTK QLKL DPSI FESL QKER VEAG DVIY IEAN SGAV KRQG RCDT YATE FDLE AEEY VPLP KGDV HKKK EIIQ DVTL
Gene ID:	8607
Uniprot:	Q9Y265
Cellular Location:	Cytoplasm, Membrane, Nucleus, Nucleus matrix, centrosome, cytoskeleton, microtubule organizing center, nucleoplasm
Calculated MW:	42kDa/50kDa
Observed MW:	55kDa

Synonyms:

RUVBL1, ECP-54, ECP54, INO80H, NMP 238, NMP238, PONTIN, Pontin52, RVB1, TIH1, TIP49, TIP49A

Background:

This gene encodes a protein that has both DNA-dependent ATPase and DNA helicase activities and belongs to the ATPases associated with diverse cellular activities (AAA+) protein family. The encoded protein associates with several multisubunit transcriptional complexes and with protein complexes involved in both ATP-dependent remodeling and histone modification. Alternate splicing results in multiple transcript variants.

UniProt Protein Function:	RUVBL1: Possesses single-stranded DNA-stimulated ATPase and ATP- dependent DNA helicase (3' to 5') activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL1 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1- CTNNB1 complex. Belongs to the ruvB family. Forms homotypic and heterotypic interactions. Forms a multimeric complex with RUVBL2. Interacts with the transcriptional activation domain of MYC. Component of the RNA polymerase II holoenzyme complex. May also act to bridge the LEF1/TCF1-CTNNB1 complex and TBP. Component of the NuA4 histone acetyltransferase complex which contains the catalytic subunit KAT5/TIP60 and the subunits EP400, TRRAP/PAF400, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, ING3, actin, ACTL6A/BAF53A, MORF4L1/MRG15, MORF4L2/MRGX, MRGBP, YEATS4/GAS41, VPS72/YL1 and MEAF6. The NuA4 complex interacts with MYC and the adenovirus E1A protein. RUVBL1 interacts with EP400. Component of a NuA4-related complex which contains EP400, TRRAP/PAF400, SRCAP, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, actin, ACTL6A/BAF53A, VPS72 and YEATS4/GAS41. Component of the BAF53 complex, at least composed of ACTL6A/BAF53A, RUVBL1/TIP49, SMARCA2/BRM, and TRRAP/PAF400. Component of some MLL1/MLL complex, at least composed of the core components MLL, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components C17orf49, CHD8, E2F6, HSP70, IN80C, KIAA1267, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Associates with alpha and gamma tubulins, particularly during metaphase and early anaphase. Interacts with NPAT. Component of the chromatin-remodeling INO80 complex, at least composed of ACTL6A, ACTR5, ACTR8, RVBL1, RVBL2, INO80, INO80B, INO80C, INO80D and INO80E. Interacts with IGHMBP2. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Helicase; EC 3.6.4.12; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 3q21 Cellular Component: nucleoplasm; Golgi apparatus; NuA4 histone acetyltransferase complex; nuclear matrix; membrane; intracellular membrane-bound organelle; cytoplasm; microtubule organizing center; nucleus Molecular Function:DNA helicase activity; protein binding; ATPase activity; ATP binding Biological Process: regulation of transcription from RNA polymerase II promoter; nucleosome assembly; mitosis; establishment and/or maintenance of chromatin architecture; DNA replication-independent nucleosome assembly at centromere; transcription, DNA-dependent; cell division; regulation of growth; spermatogenesis; DNA repair; DNA duplex unwinding; DNA recombination
UniProt Code:	Q9Y265
NCBI GenInfo Identifier:	28201891
NCBI Gene ID:	8607
NCBI Accession:	Q9Y265.1
UniProt Secondary Accession:	Q9Y265,P82276, Q1KMR0, Q53HK5, Q53HL7, Q53Y27, Q9BSX9 B2R5S0,
UniProt Related Accession:	Q9Y265
Molecular Weight:	456
NCBI Full Name:	RuvB-like 1
NCBI Synonym Full Names:	RuvB-like AAA ATPase 1
NCBI Official Symbol:	RUVBL1
NCBI Official Synonym Symbols:	RVB1; TIH1; ECP54; TIP49; INO80H; NMP238; PONTIN; TIP49A; Pontin52
NCBI Protein Information:	ruvB-like 1; ECP-54; NMP 238; pontin 52; TAP54-alpha; INO80 complex subunit H; nuclear matrix protein 238; RuvB (E coli homolog)-like 1; 49 kDa TBP-interacting protein; TIP60-associated protein 54-alpha; 54 kDa erythrocyte cytosolic protein; TATA binding protein interacting protein 49 kDa; 49 kDa TATA box-binding protein-interacting protein
UniProt Protein Name:	RuvB-like 1
UniProt Synonym Protein Names:	49 kDa TATA box-binding protein-interacting protein; 49 kDa TBP-interacting protein; 54 kDa erythrocyte cytosolic protein; ECP-54; INO80 complex subunit H; Nuclear matrix protein 238; NMP 238; Pontin 52; TIP49a; TIP60-associated protein 54-alpha; TAP54-alpha
Protein Family:	RuvB
UniProt Gene Name:	RUVBL1
UniProt Entry Name:	RUVB1_HUMAN

	Western blot analysis of extracts of various cell lines, using RUVBL1 antibody (CAB5723) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 15s.
	Immunohistochemistry of paraffin-embedded human stomach using RUVBL1 Antibody (CAB5723) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human gastric cancer using RUVBL1 Antibody (CAB5723) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using RUVBL1 Antibody (CAB5723) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of A549 cells using RUVBL1 antibody (CAB5723). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-RUVBL1 Antibody (CAB14195)	Human TIP49A ELISA Kit
Anti-TIP49A Antibody (CAB5180)	Human RuvB-like 1 (RUVBL1) ELISA Kit
RUVBL1 Antibody (PACO11992)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)