Description
RV-A VP7 Recombinant Protein (C-Strep)
RV-A VP7 Recombinant Protein (C-Strep) is a recombinant protein expressed in mammalian cells, spanning Gln51-Ile326, with a C-terminal Strep tag. Predicted molecular weight: 34.33 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Outer capsid glycoprotein VP7 is a ~37 kDa protein. Calcium-binding protein that interacts with rotavirus cell receptors once the initial attachment by VP4 has been achieved. For research use only.
Rotavirus attachment and entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors. Following entry into the host cell, low intracellular or intravesicular Ca(2+) concentration probably causes the calcium-stabilized VP7 trimers to dissociate from the virion. This step is probably necessary for the membrane-disrupting entry step and the release of VP4, which is locked onto the virion by VP7.
| Product Name: | RV-A VP7 Recombinant Protein (C-Strep) |
| SKU: | GXRP10314 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | RV-A |
| Synonyms: | Outer capsid glycoprotein VP7 |
| Tag: | C-Strep |
| Expression Host: | Mammalian cells |
| Protein Length: | Gln51-Ile326 |
| Calculated MW: | 34.33 kDa |
| UniProt: | P12476 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only |
