Anti-Protein S100-A9 Antibody (CAB9842)

Antibody Name:	Anti-Protein S100-A9 Antibody
Antibody SKU:	CAB9842
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-114 of human S100A9 (NP_002956.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse spleen, Rat spleen

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-114 of human S100A9 (NP_002956.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MTCK MSQL ERNI ETII NTFH QYSV KLGH PDTL NQGE FKEL VRKD LQNF LKKE NKNE KVIE HIME DLDT NADK QLSF EEFI MLMA RLTW ASHE KMHE GDEG PGHH HKPG LGEG TP
Gene ID:	6280
Uniprot:	P06702
Cellular Location:	Cell membrane, Cytoplasm, Peripheral membrane protein, Secreted, cytoskeleton
Calculated MW:	13kDa
Observed MW:	13kDa

Synonyms:

S100A9, 60B8AG, CAGB, CFAG, CGLB, L1AG, LIAG, MAC387, MIF, MRP14, NIF, P14

Background:

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and altered expression of this protein is associated with the disease cystic fibrosis. This antimicrobial protein exhibits antifungal and antibacterial activity.

UniProt Protein Function:	S100A9: a calcium-binding regulatory protein of the S-100 family expressed by macrophages in acutely inflammated tissues and in chronic inflammations. May be an inhibitor of protein kinases. Also expressed in epithelial cells constitutively or induced during dermatoses. May interact with components of the intermediate filaments in monocytes and epithelial cells. Interacts with CEACAM3 in a calcium-dependent manner.
UniProt Protein Details:	Protein type:Calcium-binding Chromosomal Location of Human Ortholog: 1q21 Cellular Component: cytoskeleton; cytosol; extracellular region; extracellular space; nucleus; plasma membrane Molecular Function:arachidonic acid binding; calcium ion binding; microtubule binding; protein binding; RAGE receptor binding; signal transducer activity; zinc ion binding Biological Process: activation of NF-kappaB transcription factor; autophagy; caspase activation; cell-cell signaling; chemokine production; cytokine production; defense response to bacterium; defense response to fungus; inflammatory response; leukocyte migration during inflammatory response; neutrophil chemotaxis; positive regulation of cell growth; positive regulation of inflammatory response; regulation of cytoskeleton organization and biogenesis; sequestering of zinc ion
NCBI Summary:	The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and altered expression of this protein is associated with the disease cystic fibrosis. This antimicrobial protein exhibits antifungal and antibacterial activity. [provided by RefSeq, Nov 2014]
UniProt Code:	P06702
NCBI GenInfo Identifier:	115444
NCBI Gene ID:	6280
NCBI Accession:	P06702.1
UniProt Secondary Accession:	P06702,Q6FGA1, Q9NYM0, Q9UCJ1, D3DV36,
UniProt Related Accession:	P06702
Molecular Weight:	13,242 Da
NCBI Full Name:	Protein S100-A9
NCBI Synonym Full Names:	S100 calcium binding protein A9
NCBI Official Symbol:	S100A9
NCBI Official Synonym Symbols:	MIF; NIF; P14; CAGB; CFAG; CGLB; L1AG; LIAG; MRP14; 60B8AG; MAC387
NCBI Protein Information:	protein S100-A9
UniProt Protein Name:	Protein S100-A9
UniProt Synonym Protein Names:	Calgranulin-B; Calprotectin L1H subunit; Leukocyte L1 complex heavy chain; Migration inhibitory factor-related protein 14; MRP-14; p14; S100 calcium-binding protein A9
Protein Family:	Protein
UniProt Gene Name:	S100A9
UniProt Entry Name:	S10A9_HUMAN

	Western blot analysis of extracts of Mouse spleen, using S100A9 antibody (CAB9842) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Western blot analysis of extracts of Rat spleen, using S100A9 antibody (CAB9842) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded rat spleen using S100A9 antibody (CAB9842) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human tonsil using S100A9 antibody (CAB9842) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse spleen using S100A9 antibody (CAB9842) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
S100A9 Antibody (PACO12009)	Human S100A9 ELISA Kit
	Human S100A9 (S100 Calcium Binding Protein A9) CLIA Kit (HUES00742)
	Human S100A9 (S100 Calcium Binding Protein A9) ELISA Kit (HUES02346)
	Human Protein S100-A9 (S100A9) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)