Anti-SAFB Antibody (CAB7927)

Antibody Name:	Anti-SAFB Antibody
Antibody SKU:	CAB7927
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human SAFB (NP_001188267.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, DU145, NIH/3T3, Mouse brain, Mouse lung, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human SAFB (NP_001188267.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAET LSGL GDSG AAGA AALS SASS ETGT RRLS DLRV IDLR AELR KRNV DSSG NKSV LMER LKKA IEDE GGNP DEIE ITSE GNKK TSKR SSKG RKPE EEGV EDNG LEEN SGDG QEDV ETSL ENLQ DIDI MDIS VLDE AEID NGSV ADCV EDDD ADNL QESL SDSR ELVE GEMK ELPE QLQE HAIE DKET INNL DTSS SDFT
Gene ID:	6294
Uniprot:	Q15424
Cellular Location:	Nucleus
Calculated MW:	95kDa/102kDa
Observed MW:	155kDa

Synonyms:

SAFB, HAP, HET, SAB-B1, SAF-B, SAF-B1, SAFB1

Background:

This gene encodes a DNA-binding protein which has high specificity for scaffold or matrix attachment region DNA elements (S/MAR DNA). This protein is thought to be involved in attaching the base of chromatin loops to the nuclear matrix but there is conflicting evidence as to whether this protein is a component of chromatin or a nuclear matrix protein. Scaffold attachment factors are a specific subset of nuclear matrix proteins (NMP) that specifically bind to S/MAR. The encoded protein is thought to serve as a molecular base to assemble a 'transcriptosome complex' in the vicinity of actively transcribed genes. It is involved in the regulation of heat shock protein 27 transcription, can act as an estrogen receptor co-repressor and is a candidate for breast tumorigenesis. This gene is arranged head-to-head with a similar gene whose product has the same functions. Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	SAFB1: Binds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly point to allow the formation of a 'transcriptosomal' complex (consisting of SR proteins and RNA polymerase II) coupling transcription and RNA processing. Can function as an estrogen receptor corepressor and can also bind to the HSP27 promoter and decrease its transcription. When associated with RBMX, binds to and stimulates transcription from the SREBF1 promoter. Can inhibit cell proliferation.
UniProt Protein Details:	Protein type:RNA-binding; DNA-binding; Nuclear receptor co-regulator Chromosomal Location of Human Ortholog: 19p13.3-p13.2 Cellular Component: nucleoplasm; nucleus Molecular Function:double-stranded DNA binding; protein binding Biological Process: establishment and/or maintenance of chromatin architecture
NCBI Summary:	This gene encodes a DNA-binding protein which has high specificity for scaffold or matrix attachment region DNA elements (S/MAR DNA). This protein is thought to be involved in attaching the base of chromatin loops to the nuclear matrix but there is conflicting evidence as to whether this protein is a component of chromatin or a nuclear matrix protein. Scaffold attachment factors are a specific subset of nuclear matrix proteins (NMP) that specifically bind to S/MAR. The encoded protein is thought to serve as a molecular base to assemble a 'transcriptosome complex' in the vicinity of actively transcribed genes. It is involved in the regulation of heat shock protein 27 transcription, can act as an estrogen receptor co-repressor and is a candidate for breast tumorigenesis. This gene is arranged head-to-head with a similar gene whose product has the same functions. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2011]
UniProt Code:	Q15424
NCBI GenInfo Identifier:	116242782
NCBI Gene ID:	6294
NCBI Accession:	Q15424.4
UniProt Secondary Accession:	Q15424,O60406, Q59HH8, A0AV56, B7Z5B6, B7ZLP6, F5H0H3
UniProt Related Accession:	Q15424
Molecular Weight:	102,768 Da
NCBI Full Name:	Scaffold attachment factor B1
NCBI Synonym Full Names:	scaffold attachment factor B
NCBI Official Symbol:	SAFB
NCBI Official Synonym Symbols:	HAP; HET; SAF-B; SAFB1; SAB-B1; SAF-B1
NCBI Protein Information:	scaffold attachment factor B1
UniProt Protein Name:	Scaffold attachment factor B1
UniProt Synonym Protein Names:	HSP27 estrogen response element-TATA box-binding protein; HSP27 ERE-TATA-binding protein
Protein Family:	Scaffold attachment factor
UniProt Gene Name:	SAFB
UniProt Entry Name:	SAFB1_HUMAN

	Western blot analysis of extracts of various cell lines, using SAFB antibody (CAB7927) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded rat heart using SAFB antibody (CAB7927) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human gastric cancer using SAFB antibody (CAB7927) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using SAFB antibody (CAB7927) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U-2 OS cells using SAFB antibody (CAB7927) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

Anti-SAFB Antibody (CAB15721)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)