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Anti-SARDH Antibody (CAB9541)

SKU:
CAB9541
Product Type:
Antibody
Applications:
WB
Reactivity:
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
  • Cell Biology Antibodies 12 Anti-SARDH Antibody CAB9541
  • Cell Biology Antibodies 12 Anti-SARDH Antibody CAB9541
€139 - €419
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Description

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Antibody Name:Anti-SARDH Antibody
Antibody SKU:CAB9541
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 300-560 of human SARDH (NP_009032.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:Mouse, Rat
Positive Samples:Mouse brain, Mouse liver, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 300-560 of human SARDH (NP_009032.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:RIEG IQNM PNVR DHDA SVYL RLQG DALS VGGY EANP IFWE EVSD KFAF GLFD LDWE VFTQ HIEG AINR VPVL EKTG IKST VCGP ESFT PDHK PLMG EAPE LRGF FLGC GFNS AGMM LGGG CGQE LAHW IIHG RPEK DMHG YDIR RFHH SLTD HPRW IRER SHES YAKN YSVV FPHD EPLA GRNM RRDP LHEE LLGQ GCVF QERH GWER PGWF HPRG PAPV LEYD YYGA YGSR AHED YAYR RLLA DEYT FAFP PHHD TIKK E
Gene ID:1757
Uniprot:Q9UL12
Cellular Location:Mitochondrion matrix
Calculated MW:82kDa/101kDa
Observed MW:101kDa
Synonyms:SARDH, BPR-2, DMGDHL1, SAR, SARD, SDH
Background:This gene encodes an enzyme localized to the mitochondrial matrix which catalyzes the oxidative demethylation of sarcosine. This enzyme is distinct from another mitochondrial matrix enzyme, dimethylglycine dehydrogenase, which catalyzes a reaction resulting in the formation of sarcosine. Mutations in this gene are associated with sarcosinemia. Alternatively spliced transcript variants have been described.
UniProt Protein Function:SARDH: Defects in SARDH are a cause of sarcosinemia (SAR). Sarcosinemia is a metabolic disorder characterized by an increased concentration of sarcosine in plasma and urine due to sarcosine dehydrogenase deficiency. Prevalence has been estimated at 1:28'000 to 1:350'000 in newborn screening programs. Sarcosinemia is most probably a benign condition without significant clinical problems. It is transmitted in an autosomal recessive manner. Belongs to the GcvT family.
UniProt Protein Details:

Protein type:Amino Acid Metabolism - glycine, serine and threonine; Oxidoreductase; EC 1.5.8.3; Mitochondrial

Chromosomal Location of Human Ortholog: 9q33-q34

Cellular Component: mitochondrion; mitochondrial matrix

Molecular Function:sarcosine dehydrogenase activity

Disease: Sarcosinemia

NCBI Summary:This gene encodes an enzyme localized to the mitochondrial matrix which catalyzes the oxidative demethylation of sarcosine. This enzyme is distinct from another mitochondrial matrix enzyme, dimethylglycine dehydrogenase, which catalyzes a reaction resulting in the formation of sarcosine. Mutations in this gene are associated with sarcosinemia. Alternatively spliced transcript variants have been described. [provided by RefSeq, Oct 2008]
UniProt Code:Q9UL12
NCBI GenInfo Identifier:21361378
NCBI Gene ID:1757
NCBI Accession:NP_009032.2
UniProt Related Accession:Q9UL12
Molecular Weight:105kDa
NCBI Full Name:sarcosine dehydrogenase, mitochondrial
NCBI Synonym Full Names:sarcosine dehydrogenase
NCBI Official Symbol:SARDH  
NCBI Official Synonym Symbols:SAR; SDH; SARD; BPR-2; DMGDHL1  
NCBI Protein Information:sarcosine dehydrogenase, mitochondrial
UniProt Protein Name:Sarcosine dehydrogenase, mitochondrial
UniProt Synonym Protein Names:BPR-2
Protein Family:Sarcosine dehydrogenase
UniProt Gene Name:SARDH  
UniProt Entry Name:SARDH_HUMAN
Anti-SARDH Polyclonal Antibody (CAB9541)Western blot analysis of extracts of various cell lines, using SARDH Antibody (CAB9541) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-SARDH Polyclonal Antibody (CAB9541)Immunofluorescence analysis of L929 cells using Anti-SARDH Polyclonal Antibody (CAB9541) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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