Anti-SART1 Antibody (CAB8569)

Antibody Name:	Anti-SART1 Antibody
Antibody SKU:	CAB8569
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-255 of human SART1 (NP_005137.1).

Application:	WB IP
Recommended Dilution:	WB 1:500 - 1:2000 IP 1:20 - 1:50
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, Jurkat, HepG2, Mouse liver, Mouse brain, Mouse testis, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-255 of human SART1 (NP_005137.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MGSS KKHR GEKE AAGT TAAA GTGG ATEQ PPRH REHK KHKH RSGG SGGS GGER RKRS RERG GERG SGRR GAEA EARS STHG RERS QAEP SERR VKRE KRDD GYEA AASS KTSS GDAS SLSI EETN KLRA KLGL KPLE VNAI KKEA GTKE EPVT ADVI NPMA LRQR EELR EKLA AAKE KRLL NQKL GKIK TLGE DDPW LDDT AAWI ERSR QLQK EKDL AEKR AKLL EEMD QEFG VSTL VEEE FGQR RQDL YSAR DLQ
Gene ID:	9092
Uniprot:	O43290
Cellular Location:	Nucleus
Calculated MW:	90kDa
Observed MW:	120kDa

Synonyms:

SART1, Ara1, HOMS1, SART1259, SNRNP110, Snu66, HAF

Background:

This gene encodes two proteins, the SART1(800) protein expressed in the nucleus of the majority of proliferating cells, and the SART1(259) protein expressed in the cytosol of epithelial cancers. The SART1(259) protein is translated by the mechanism of -1 frameshifting during posttranscriptional regulation; its full-length sequence is not published yet. The two encoded proteins are thought to be involved in the regulation of proliferation. Both proteins have tumor-rejection antigens. The SART1(259) protein possesses tumor epitopes capable of inducing HLA-A2402-restricted cytotoxic T lymphocytes in cancer patients. This SART1(259) antigen may be useful in specific immunotherapy for cancer patients and may serve as a paradigmatic tool for the diagnosis and treatment of patients with atopy. The SART1(259) protein is found to be essential for the recruitment of the tri-snRNP to the pre-spliceosome in the spliceosome assembly pathway.

UniProt Protein Function:	SART1: May play a role in mRNA splicing. May also bind to DNA. Belongs to the SNU66/SART1 family. 1 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:RNA-binding; RNA processing; RNA splicing; Apoptosis; Spliceosome Chromosomal Location of Human Ortholog: 11q13.1 Cellular Component: nucleoplasm; Cajal body; Golgi apparatus; U4/U6 x U5 tri-snRNP complex; cytoplasm; cytosol Molecular Function:protein binding Biological Process: nuclear mRNA splicing, via spliceosome; spliceosomal snRNP biogenesis; positive regulation of cytotoxic T cell differentiation; maturation of 5S rRNA; nuclear mRNA cis splicing, via U2-type spliceosome; cell cycle arrest
NCBI Summary:	This gene encodes two proteins, the SART1(800) protein expressed in the nucleus of the majority of proliferating cells, and the SART1(259) protein expressed in the cytosol of epithelial cancers. The SART1(259) protein is translated by the mechanism of -1 frameshifting during posttranscriptional regulation; its full-length sequence is not published yet. The two encoded proteins are thought to be involved in the regulation of proliferation. Both proteins have tumor-rejection antigens. The SART1(259) protein possesses tumor epitopes capable of inducing HLA-A2402-restricted cytotoxic T lymphocytes in cancer patients. This SART1(259) antigen may be useful in specific immunotherapy for cancer patients and may serve as a paradigmatic tool for the diagnosis and treatment of patients with atopy. The SART1(259) protein is found to be essential for the recruitment of the tri-snRNP to the pre-spliceosome in the spliceosome assembly pathway. [provided by RefSeq, Jul 2008]
UniProt Code:	O43290
NCBI GenInfo Identifier:	10863889
NCBI Gene ID:	9092
NCBI Accession:	NP_005137.1
UniProt Secondary Accession:	O43290,Q53GB5, A6NDN1,
UniProt Related Accession:	O43290
Molecular Weight:	90,255 Da
NCBI Full Name:	U4/U6.U5 tri-snRNP-associated protein 1
NCBI Synonym Full Names:	squamous cell carcinoma antigen recognized by T cells
NCBI Official Symbol:	SART1
NCBI Official Synonym Symbols:	Ara1; HOMS1; Snu66; SART1259; SNRNP110
NCBI Protein Information:	U4/U6.U5 tri-snRNP-associated protein 1; SART-1; hSnu66; hSART-1; SNU66 homolog; IgE autoantigen; SART1(259) protein; SART1(800) protein; U4/U6.U5 tri-snRNP-associated 110 kDa protein; small nuclear ribonucleoprotein 110kDa (U4/U6.U5); squamous cell carcinoma antigen recognised by T cells; squamous cell carcinoma antigen recognized by T cells 1; squamous cell carcinoma antigen recognized by T-cells 1
UniProt Protein Name:	U4/U6.U5 tri-snRNP-associated protein 1
UniProt Synonym Protein Names:	SNU66 homolog; hSnu66; Squamous cell carcinoma antigen recognized by T-cells 1; SART-1; hSART-1; U4/U6.U5 tri-snRNP-associated 110 kDa protein; Allergen: Hom s 1
Protein Family:	U4/U6.U5 tri-snRNP-associated protein
UniProt Gene Name:	SART1
UniProt Entry Name:	SNUT1_HUMAN

	Western blot analysis of extracts of various cell lines, using SART1 antibody (CAB8569) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunoprecipitation analysis of 150ug extracts of Jurkat cells using 3ug SART1 antibody (CAB8569). Western blot was performed from the immunoprecipitate using SART1 antibody (CAB8569) at a dilition of 1:1000.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)