Anti-SCYL1 Antibody (CAB6735)

Antibody Name:	Anti-SCYL1 Antibody
Antibody SKU:	CAB6735
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 460-740 of human SCYL1 (NP_065731.3).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, Mouse pancreas

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 460-740 of human SCYL1 (NP_065731.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SAST RHRV LTSA FSRA TRDP FAPS RVAG VLGF AATH NLYS MNDC AQKI LPVL CGLT VDPE KSVR DQAF KAIR SFLS KLES VSED PTQL EEVE KDVH AASS PGMG GAAA SWAG WAVT GVSS LTSK LIRS HPTT APTE TNIP QRPT PEGV PAPA PTPV PATP TTSG HWET QEED KDTA EDSS TADR WDDE DWGS LEQE AESV LAQQ DDWS TGGQ VSRA SQVS NSDH KSSK SPES DWSS WEAE GSWE QGWQ EPSS QEPP PDGT RLAS EYNW GGPE SSDK GDPF A
Gene ID:	57410
Uniprot:	Q96KG9
Cellular Location:	Cytoplasm, Endoplasmic reticulum-Golgi intermediate compartment, Golgi apparatus, Nucleus, centrosome, cis-Golgi network, cytoskeleton, microtubule organizing center
Calculated MW:	69kDa/78kDa/86kDa/88kDa/89kDa
Observed MW:	110kDa

Synonyms:

SCYL1, GKLP, HT019, NKTL, NTKL, P105, SCAR21, TAPK, TEIF, TRAP

Background:

This gene encodes a transcriptional regulator belonging to the SCY1-like family of kinase-like proteins. The protein has a divergent N-terminal kinase domain that is thought to be catalytically inactive, and can bind specific DNA sequences through its C-terminal domain. It activates transcription of the telomerase reverse transcriptase and DNA polymerase beta genes. The protein has been localized to the nucleus, and also to the cytoplasm and centrosomes during mitosis. Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	SCYL1: Regulates COPI-mediated retrograde traffic. Has no detectable kinase activity in vitro. Belongs to the protein kinase superfamily. 6 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Kinase, protein; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Other group; SCY1 family Chromosomal Location of Human Ortholog: 11q13 Cellular Component: Golgi apparatus; cis-Golgi network; membrane; cytoplasm; ER-Golgi intermediate compartment; microtubule organizing center; COPI vesicle coat; nucleus Molecular Function:DNA binding; protein-tyrosine kinase activity; ATP binding Biological Process: peptidyl-tyrosine phosphorylation; regulation of transcription, DNA-dependent; transcription, DNA-dependent; retrograde vesicle-mediated transport, Golgi to ER
NCBI Summary:	This gene encodes a transcriptional regulator belonging to the SCY1-like family of kinase-like proteins. The protein has a divergent N-terminal kinase domain that is thought to be catalytically inactive, and can bind specific DNA sequences through its C-terminal domain. It activates transcription of the telomerase reverse transcriptase and DNA polymerase beta genes. The protein has been localized to the nucleus, and also to the cytoplasm and centrosomes during mitosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	Q96KG9
NCBI GenInfo Identifier:	74762671
NCBI Gene ID:	57410
NCBI Accession:	Q96KG9.1
UniProt Secondary Accession:	Q96KG9,Q96G50, Q96KG8, Q96KH1, Q9HAW5, Q9HBL3, Q9NR53 A6NJF1,
UniProt Related Accession:	Q96KG9
Molecular Weight:	808
NCBI Full Name:	N-terminal kinase-like protein
NCBI Synonym Full Names:	SCY1-like 1 (S. cerevisiae)
NCBI Official Symbol:	SCYL1
NCBI Official Synonym Symbols:	GKLP; NKTL; NTKL; P105; TAPK; TEIF; TRAP; HT019
NCBI Protein Information:	N-terminal kinase-like protein; SCY1-like protein 1; teratoma-associated tyrosine kinase; telomerase regulation-associated protein; coated vesicle-associated kinase of 90 kDa; telomerase transcriptional element-interacting factor; telomerase transcriptional elements-interacting factor; likely ortholog of mouse N-terminal kinase-like protein
UniProt Protein Name:	N-terminal kinase-like protein
UniProt Synonym Protein Names:	Coated vesicle-associated kinase of 90 kDa; SCY1-like protein 1; Telomerase regulation-associated protein; Telomerase transcriptional element-interacting factor; Teratoma-associated tyrosine kinase
Protein Family:	N-terminal kinase-like protein
UniProt Gene Name:	SCYL1
UniProt Entry Name:	NTKL_HUMAN

	Western blot analysis of extracts of various cell lines, using SCYL1 Antibody (CAB6735) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunohistochemistry of paraffin-embedded human liver damage using SCYL1 antibody (CAB6735) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat kidney using SCYL1 antibody (CAB6735) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of MCF-7 cells using SCYL1 antibody (CAB6735). Blue: DAPI for nuclear staining.

Antibodies

Anti-SCYL1 Antibody (CAB19913)[KO Validated]

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)