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Anti-SEC11A Antibody (CAB10552)

SKU:
CAB10552
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
  • Cell Biology Antibodies 2 Anti-SEC11A Antibody CAB10552
  • Cell Biology Antibodies 2 Anti-SEC11A Antibody CAB10552
  • Cell Biology Antibodies 2 Anti-SEC11A Antibody CAB10552
€139 - €419
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Description

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Antibody Name:Anti-SEC11A Antibody
Antibody SKU:CAB10552
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 37-145 of human SEC11A (NP_055115.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:SGC-7901, HT-29, Raji, HepG2, A-549, Mouse kidney, Mouse liver, Mouse lung, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 37-145 of human SEC11A (NP_055115.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:KGLM VITG SESP IVVV LSGS MEPA FHRG DLLF LTNR VEDP IRVG EIVV FRIE GREI PIVH RVLK IHEK QNGH IKFL TKGD NNAV DDRG LYKQ GQHW LEKK DVVG RARG F
Gene ID:23478
Uniprot:P67812
Cellular Location:Endoplasmic reticulum membrane, Microsome membrane, Single-pass type II membrane protein
Calculated MW:17kDa/18kDa/20kDa/21kDa
Observed MW:21kDa
Synonyms:SEC11A, 1810012E07Rik, SEC11L1, SPC18, SPCS4A, sid2895
Background:This gene encodes a member of the peptidase S26B family. The encoded protein is an 18kDa subunit of the signal peptidase complex and has been linked to cell migration and invasion, gastric cancer and lymph node metastasis. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 8.
UniProt Protein Function:SEC11A: Component of the microsomal signal peptidase complex which removes signal peptides from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum. Belongs to the peptidase S26B family.
UniProt Protein Details:

Protein type:EC 3.4.21.89; Membrane protein, integral; Protease

Chromosomal Location of Human Ortholog: 15q25.2-q25.3

Cellular Component: endoplasmic reticulum membrane; signal peptidase complex

Molecular Function:peptidase activity

Biological Process: signal peptide processing

NCBI Summary:This gene encodes a member of the peptidase S26B family. The encoded protein is an 18kDa subunit of the signal peptidase complex and has been linked to cell migration and invasion, gastric cancer and lymph node metastasis. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 8. [provided by RefSeq, Dec 2012]
UniProt Code:P67812
NCBI GenInfo Identifier:54039634
NCBI Gene ID:23478
NCBI Accession:P67812.1
UniProt Secondary Accession:P67812,O75957, P21378, Q53FQ8, B2RAD7, B4DUL4, H0YK72 H0YK83,
UniProt Related Accession:P67812
Molecular Weight:21kDa
NCBI Full Name:Signal peptidase complex catalytic subunit SEC11A
NCBI Synonym Full Names:SEC11 homolog A, signal peptidase complex subunit
NCBI Official Symbol:SEC11A  
NCBI Official Synonym Symbols:SPC18; SPCS4A; SEC11L1; sid2895; 1810012E07Rik  
NCBI Protein Information:signal peptidase complex catalytic subunit SEC11A
UniProt Protein Name:Signal peptidase complex catalytic subunit SEC11A
UniProt Synonym Protein Names:Endopeptidase SP18; Microsomal signal peptidase 18 kDa subunit; SPase 18 kDa subunit; SEC11 homolog A; SEC11-like protein 1; SPC18
Protein Family:Signal peptidase complex
UniProt Gene Name:SEC11A  
Anti-SEC11A Antibody (CAB10552)Western blot analysis of extracts of various cell lines, using SEC11A antibody (CAB10552) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-SEC11A Antibody (CAB10552)Immunofluorescence analysis of L929 cells using SEC11A Rabbit pAb (CAB10552) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-SEC11A Antibody (CAB10552)Immunofluorescence analysis of U2OS cells using SEC11A Rabbit pAb (CAB10552) at dilution of 1:100. Blue: DAPI for nuclear staining.

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