The Sec61B Polyclonal Antibody (CAB10007) is a valuable tool for researchers studying Sec61B, a key protein involved in protein translocation across the endoplasmic reticulum membrane. This antibody, produced in rabbits, exhibits high specificity for human samples and is validated for use in Western blotting applications. By targeting the Sec61B protein, this antibody enables accurate detection and analysis of protein translocation processes in various cell types, making it essential for studies in molecular biology and cell biology.Sec61B is a crucial component of the protein translocation machinery, playing a fundamental role in protein synthesis and trafficking within the cell. Understanding the function and regulation of Sec61B is essential for unraveling the mechanisms underlying cellular homeostasis and protein quality control.
Research involving Sec61B has implications for a wide range of biological processes, including protein folding, protein sorting, and the unfolded protein response.By offering a reliable tool for the specific detection of Sec61B, the Sec61B Polyclonal Antibody facilitates in-depth investigations into protein translocation dynamics and cellular processes that depend on proper protein trafficking. Researchers can leverage this antibody to elucidate the role of Sec61B in various cellular pathways, paving the way for new insights into cell physiology and potential therapeutic targets for diseases related to protein misfolding and trafficking defects.
Product Name:
SEC61B Polyclonal Antibody
SKU:
CAB21807
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-96 of human SEC61B (NP_006799.1).
The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. Oligomers of the Sec61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane. This complex consists of three membrane proteins- alpha, beta, and gamma. This gene encodes the beta-subunit protein. The Sec61 subunits are also observed in the post-ER compartment, suggesting that these proteins can escape the ER and recycle back. There is evidence for multiple polyadenylated sites for this transcript.
Purification Method:
Affinity purification
Gene ID:
10952
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using SEC61B Rabbit pAb (CAB21807) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
