Western blot analysis of Jurkat, using SETD3 Rabbit pAb (CAB21681) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The SETD3 Polyclonal Antibody (CAB21681) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, demonstrates high reactivity with human samples and is validated for use in Western blot applications.SETD3 is known for its role in regulating actin dynamics, which in turn influences cellular processes such as cell migration, division, and signaling. By targeting the SETD3 protein, researchers can gain valuable insights into the molecular mechanisms underlying these processes and their relevance in various biological pathways.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
SETD3 Polyclonal Antibody
SKU:
CAB21681
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
hSETD3, C14orf154, SETD3
Positive Sample:
Jurkat
Cellular Localization:
Nucleus.
Calculated MW:
67kDa
Observed MW:
67kDa
Enables protein-L-histidine N-tele-methyltransferase activity. Involved in actin modification and peptidyl-histidine methylation. Located in cytoplasm.
Purification Method
Affinity purification
Gene ID
84193
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Jurkat cells, using SETD3 Rabbit pAb (CAB21681) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 45s.
Immunoprecipitation analysis of 200 μg extracts of HeLa cells, using 3 μg SETD3 antibody (CAB21681). Western blot was performed from the immunoprecipitate using SETD3 antibody (CAB21681) at a dilution of 1:1000.
