The SETD3 Polyclonal Antibody (CAB21681) is a high-quality antibody developed for reliable detection and analysis of target proteins. Enables protein-L-histidine N-tele-methyltransferase activity. Involved in actin modification and peptidyl-histidine methylation. Located in cytoplasm.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
SETD3 Polyclonal Antibody
SKU:
CAB21681
Size:
100μL, 20μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIPELISA
Recommended Dilution:
WB
1:500 - 1:2000
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
hSETD3, C14orf154, SETD3
Positive Sample:
Jurkat
Cellular Localization:
Nucleus.
Calculated MW:
67kDa
Observed MW:
67kDa
Enables protein-L-histidine N-tele-methyltransferase activity. Involved in actin modification and peptidyl-histidine methylation. Located in cytoplasm.
Purification Method
Affinity purification
Gene ID
84193
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Jurkat cells, using SETD3 Rabbit pAb (CAB21681) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 45s.
Immunoprecipitation analysis of 200 μg extracts of HeLa cells, using 3 μg SETD3 antibody (CAB21681). Western blot was performed from the immunoprecipitate using SETD3 antibody (CAB21681) at a dilution of 1:1000.
