Anti-SF3B2 Antibody (CAB5875)

Antibody Name:	Anti-SF3B2 Antibody
Antibody SKU:	CAB5875
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 616-895 of human SF3B2 (NP_006833.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, MCF7, SW620, A375, Mouse testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 616-895 of human SF3B2 (NP_006833.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SLGM PVGP NAHK VPPP WLIA MQRY GPPP SYPN LKIP GLNS PIPE SCSF GYHA GGWG KPPV DETG KPLY GDVF GTNA AEFQ TKTE EEEI DRTP WGEL EPSD EESS EEEE EEES DEDK PDET GFIT PADS GLIT PGGF SSVP AGME TPEL IELR KKKI EEAM DGSE TPQL FTVL PEKR TATV GGAM MGST HIYD MSTV MSRK GPAP ELQG VEVA LAPE ELEL DPMA MTQK YEEH VREQ QAQV EKED FSDM VAEH AAKQ KQKK RKAQ PQDS RGGS KKYK EFKF
Gene ID:	10992
Uniprot:	Q13435
Cellular Location:	Nucleus
Calculated MW:	100kDa
Observed MW:	140kDa

Synonyms:

SF3B2, Cus1, SAP145, SF3B145, SF3b1, SF3b150

Background:

This gene encodes subunit 2 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 2 associates with pre-mRNA upstream of the branch site at the anchoring site. Subunit 2 also interacts directly with subunit 4 of the splicing factor 3b complex. Subunit 2 is a highly hydrophilic protein with a proline-rich N-terminus and a glutamate-rich stretch in the C-terminus.

UniProt Protein Function:	SF3B2: subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (bps) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.
UniProt Protein Details:	Protein type:RNA-binding; RNA splicing; Spliceosome; RNA processing Chromosomal Location of Human Ortholog: 11q13.1 Cellular Component: nucleoplasm; snRNP U2; spliceosome; U12-dependent spliceosome Molecular Function:protein binding Biological Process: mRNA processing; nuclear mRNA splicing, via spliceosome; RNA splicing
NCBI Summary:	This gene encodes subunit 2 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 2 associates with pre-mRNA upstream of the branch site at the anchoring site. Subunit 2 also interacts directly with subunit 4 of the splicing factor 3b complex. Subunit 2 is a highly hydrophilic protein with a proline-rich N-terminus and a glutamate-rich stretch in the C-terminus. [provided by RefSeq, Jul 2008]
UniProt Code:	Q13435
NCBI GenInfo Identifier:	296452908
NCBI Gene ID:	10992
NCBI Accession:	Q13435.2
UniProt Secondary Accession:	Q13435,Q7L4T5, Q7Z627, Q969K1, Q96CM6, Q9BWD2, A8K485 B4DT19,
UniProt Related Accession:	Q13435
Molecular Weight:	100,228 Da
NCBI Full Name:	Splicing factor 3B subunit 2
NCBI Synonym Full Names:	splicing factor 3b subunit 2
NCBI Official Symbol:	SF3B2
NCBI Official Synonym Symbols:	Cus1; SF3b1; SAP145; SF3B145; SF3b150
NCBI Protein Information:	splicing factor 3B subunit 2
UniProt Protein Name:	Splicing factor 3B subunit 2
UniProt Synonym Protein Names:	Pre-mRNA-splicing factor SF3b 145 kDa subunit; SF3b145; SF3b150; Spliceosome-associated protein 145; SAP 145
Protein Family:	Splicing factor
UniProt Gene Name:	SF3B2
UniProt Entry Name:	SF3B2_HUMAN

	Western blot analysis of extracts of various cell lines, using SF3B2 antibody (CAB5875) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunohistochemistry of paraffin-embedded rat heart using SF3B2 antibody (CAB5875) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human lung cancer using SF3B2 antibody (CAB5875) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse Intestine using SF3B2 antibody (CAB5875) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of A-549 cells using SF3B2 antibody (CAB5875).

Antibodies

SF3B2 Antibody (PACO12135)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)